Anti-CAMKIV antibody (ab3557)
Key features and details
- Rabbit polyclonal to CAMKIV
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CAMKIV antibody
See all CAMKIV primary antibodies -
Description
Rabbit polyclonal to CAMKIV -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide corresponding to Mouse CAMKIV aa 127-143.
Sequence:VEKGYYSERDAADAVKQ
(Peptide available asab5859)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. |
| Notes |
|---|
|
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. |
Target
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Function
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory. -
Tissue specificity
Expressed in epithelial ovarian cancer tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. -
Cellular localization
Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix. - Information by UniProt
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Database links
- Entrez Gene: 814 Human
- Entrez Gene: 25050 Rat
- Omim: 114080 Human
- SwissProt: Q16566 Human
- SwissProt: P13234 Rat
- Unigene: 591269 Human
- Unigene: 11046 Rat
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Alternative names
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
see all
Images
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Western blot - Anti-CAMKIV antibody (ab3557)
ab3557 at a 0.5µg/ml concentration staining 55 kDa CaMKIV in rat brain extract by Western blot.
Protocols
Datasheets and documents
References (9)
ab3557 has been referenced in 9 publications.
- Zhang XZ et al. Mechanism of AMPK-mediated apoptosis of rat gastric smooth muscle cells under high glucose condition. Biosci Rep 39:N/A (2019). PubMed: 31769476
- Park D & Chang S Soluble Aß1-42increases the heterogeneity in synaptic vesicle pool size among synapses by suppressing intersynaptic vesicle sharing. Mol Brain 11:10 (2018). PubMed: 29463281
- Ramos-Fernández E et al. Wnt-7a Stimulates Dendritic Spine Morphogenesis and PSD-95 Expression Through Canonical Signaling. Mol Neurobiol N/A:N/A (2018). PubMed: 29967987
- Chen CM et al. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice. Proc Natl Acad Sci U S A 114:E619-E628 (2017). PubMed: 28069946
- Kang X et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . PubMed: 25915125
- Zheng Z et al. Oligomeric amyloid-{beta} inhibits the proteolytic conversion of brain-derived neurotrophic factor (BDNF), AMPA receptor trafficking, and classical conditioning. J Biol Chem 285:34708-17 (2010). WB ; Turtle . PubMed: 20807770
- Toyoda H et al. Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice. Mol Brain 3:27 (2010). IHC . PubMed: 20846411
- Zheng Z & Keifer J PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning. J Neurophysiol 101:2539-49 (2009). WB ; Turtle . PubMed: 19261706
- Zhang X et al. Calcium/calmodulin-dependent protein kinase (CaMK) IV mediates nucleocytoplasmic shuttling and release of HMGB1 during lipopolysaccharide stimulation of macrophages. J Immunol 181:5015-23 (2008). WB, ICC/IF ; Human . PubMed: 18802105
