- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-CAMKIV antibody
See all CAMKIV primary antibodies -
DescriptionRabbit polyclonal to CAMKIV
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Dog, Human, Xenopus laevis, Non human primates, Plants, Turtle
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Immunogen
Synthetic peptide corresponding to Mouse CAMKIV aa 127-143.
Sequence:VEKGYYSERDAADAVKQ
(Peptide available asab5859) -
Positive control
- WB: HeLa cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab3557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).Can be blocked with CAMKIV peptide (ab5859). This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. |
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| ICC/IF | Use at an assay dependent concentration. PubMed: 18802105 |
Target
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FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
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Tissue specificityExpressed in epithelial ovarian cancer tissue.
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Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. -
Cellular localizationCytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
- Information by UniProt
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Database links
- Entrez Gene: 814 Human
- Entrez Gene: 12326 Mouse
- Entrez Gene: 25050 Rat
- Omim: 114080 Human
- SwissProt: Q16566 Human
- SwissProt: P08414 Mouse
- SwissProt: P13234 Rat
- Unigene: 591269 Human
see all -
Alternative names
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
see all
Images
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Western blot - Anti-CAMKIV antibody (ab3557)ab3557 at a 0.5µg/ml concentration staining 55 kDa CaMKIV in rat brain extract by Western blot.
Protocols
References
This product has been referenced in:
- Park D & Chang S Soluble Aß1-42increases the heterogeneity in synaptic vesicle pool size among synapses by suppressing intersynaptic vesicle sharing. Mol Brain 11:10 (2018). Read more (PubMed: 29463281) »
- Chen CM et al. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice. Proc Natl Acad Sci U S A 114:E619-E628 (2017). Read more (PubMed: 28069946) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"