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Neuroscience Neurotransmission Calcium Signaling Calmodulin / CaMK
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Anti-CAMKIV antibody (ab3557)

  • Datasheet
Reviews (3) Submit a question References (9)

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Western blot - Anti-CAMKIV antibody (ab3557)

    Key features and details

    • Rabbit polyclonal to CAMKIV
    • Suitable for: WB
    • Reacts with: Rat, Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CAMKIV antibody
      See all CAMKIV primary antibodies
    • Description

      Rabbit polyclonal to CAMKIV
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Mouse CAMKIV aa 127-143.
      Sequence:

      VEKGYYSERDAADAVKQ


      (Peptide available as ab5859)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Calcium Signaling
      • Calmodulin / CaMK
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Phosphorylation
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Calmodulin Pathway

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant Human CAMKIV protein (ab119450)
    • Recombinant Protein

      • Recombinant Human CAMKIV protein (ab60756)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3557 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Rat
    Application Abreviews Notes
    WB (3)
    Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).

    This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate.

    Notes
    WB
    Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).

    This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate.

    Target

    • Function

      Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
    • Tissue specificity

      Expressed in epithelial ovarian cancer tissue.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
    • Cellular localization

      Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
    • Target information above from: UniProt accession Q16566 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 814 Human
      • Entrez Gene: 25050 Rat
      • Omim: 114080 Human
      • SwissProt: Q16566 Human
      • SwissProt: P13234 Rat
      • Unigene: 591269 Human
      • Unigene: 11046 Rat
      • Alternative names

        • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
        • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
        • Brain Ca++-calmodulin dependent protein kinase type IV antibody
        • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
        • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
        • Calcium/calmodulin dependent protein kinase IV antibody
        • Calcium/calmodulin dependent protein kinase type IV antibody
        • Calcium/calmodulin-dependent protein kinase type IV antibody
        • CAM kinase 4 antibody
        • CAM kinase GR antibody
        • CAM kinase IV antibody
        • CAM kinase-GR antibody
        • CaMK 4 antibody
        • CAMK GR antibody
        • CaMK IV antibody
        • Camk4 antibody
        • CaMKGR antibody
        • IV antibody
        • KCC4_HUMAN antibody
        • MGC36771 antibody
        see all

      Images

      • Western blot - Anti-CAMKIV antibody (ab3557)
        Western blot - Anti-CAMKIV antibody (ab3557)

        ab3557 at a 0.5µg/ml concentration staining 55 kDa CaMKIV in rat brain extract by Western blot.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (9)

      Publishing research using ab3557? Please let us know so that we can cite the reference in this datasheet.

      ab3557 has been referenced in 9 publications.

      • Zhang XZ  et al. Mechanism of AMPK-mediated apoptosis of rat gastric smooth muscle cells under high glucose condition. Biosci Rep 39:N/A (2019). PubMed: 31769476
      • Park D & Chang S Soluble Aß1-42increases the heterogeneity in synaptic vesicle pool size among synapses by suppressing intersynaptic vesicle sharing. Mol Brain 11:10 (2018). PubMed: 29463281
      • Ramos-Fernández E  et al. Wnt-7a Stimulates Dendritic Spine Morphogenesis and PSD-95 Expression Through Canonical Signaling. Mol Neurobiol N/A:N/A (2018). PubMed: 29967987
      • Chen CM  et al. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice. Proc Natl Acad Sci U S A 114:E619-E628 (2017). PubMed: 28069946
      • Kang X  et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . PubMed: 25915125
      • Zheng Z  et al. Oligomeric amyloid-{beta} inhibits the proteolytic conversion of brain-derived neurotrophic factor (BDNF), AMPA receptor trafficking, and classical conditioning. J Biol Chem 285:34708-17 (2010). WB ; Turtle . PubMed: 20807770
      • Toyoda H  et al. Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice. Mol Brain 3:27 (2010). IHC . PubMed: 20846411
      • Zheng Z & Keifer J PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning. J Neurophysiol 101:2539-49 (2009). WB ; Turtle . PubMed: 19261706
      • Zhang X  et al. Calcium/calmodulin-dependent protein kinase (CaMK) IV mediates nucleocytoplasmic shuttling and release of HMGB1 during lipopolysaccharide stimulation of macrophages. J Immunol 181:5015-23 (2008). WB, ICC/IF ; Human . PubMed: 18802105

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-CAMKIV antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (EL4)
      Loading amount
      10000 µg
      Specification
      EL4
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 04 2012

      Western blot abreview for Anti-CAMKIV antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Zebrafish Tissue lysate - whole (ZF embryo)
      Loading amount
      30 µg
      Specification
      ZF embryo
      Treatment
      de-yolked
      Gel Running Conditions
      Non-reduced Denaturing (10% SDS)
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
      Read More

      Ms. Gemma Lace

      Verified customer

      Submitted Jan 15 2010

      Western blot abreview for Anti-CAMKIV antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (huh-7 cells)
      Loading amount
      10000 cells
      Specification
      huh-7 cells
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 26 2008

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