Overview

  • Product name

    Anti-CAMKIV antibody [EP2564Y]
    See all CAMKIV primary antibodies
  • Description

    Rabbit monoclonal [EP2564Y] to CAMKIV
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CAMKIV aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899) and human brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab75874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
    • Tissue specificity

      Expressed in epithelial ovarian cancer tissue.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
    • Cellular localization

      Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
      • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
      • Brain Ca++-calmodulin dependent protein kinase type IV antibody
      • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
      • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
      • Calcium/calmodulin dependent protein kinase IV antibody
      • Calcium/calmodulin dependent protein kinase type IV antibody
      • Calcium/calmodulin-dependent protein kinase type IV antibody
      • CAM kinase 4 antibody
      • CAM kinase GR antibody
      • CAM kinase IV antibody
      • CAM kinase-GR antibody
      • CaMK 4 antibody
      • CAMK GR antibody
      • CaMK IV antibody
      • Camk4 antibody
      • CaMKGR antibody
      • IV antibody
      • KCC4_HUMAN antibody
      • MGC36771 antibody
      see all

    Images

    • All lanes : Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 52 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27611779) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your reply.
    Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these additional two antibodies:
    ab13898
    ab28669
    I hope this will be helpful. I look forward to receiving your confirmation that you would like to go ahead, and I will be pleased to arrange and send the discount codes to you.
    For the remaining antibodies, I can suggest you may like to consider submitting an Abreview anyway, for which you can obtain Abpoints:
    ab85612
    ab75874
    ab61113
    ab47504
    ab16476
    ab85608
    ab31232
    ab41172
    ab23832
    Thank you for your time and continued interest in our products. I look forward to hearing from you.

    Read More

    Answer

    Thank you very much for your interest in our antibodies.

    We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

    If a customer would like to test an antibody in an application or species that it has not been previously tested in, we are able to provide our testing discount program.

    In this case, the antibodiess you have listed have all been tested in western blot already, but we would be able to provide this offer to you to test the following antibodies in mouse and rat:

    Discount to detect in mouse and rat (the following antibodies have not yet been tested in mouse or rat):
    CDC42 Binding Protein Kinase Beta antibody ab61328
    CNKR2 antibody ab64844
    NEK7 antibody ab80948

    Discount to test in mouse (the following antibodies have not yet been tested in mouse):
    MEK3 antibody ab5428
    BMX antibody ab73887
    Muscarinic Acetylcholine Receptor 1 antibody ab75178

    Discount to test in rat (the following antibodies have not been tested in rat):
    PPP4C antibody sb70888

    The following are already tested in western blot, and in mouse and/or rat as per your list. I am sorry we are therefore not able to provide a testing discount for these.
    ROCK2 antibody ab85612 Already tested WB, ms and rat
    CAMKIV antibody [EP2564Y] ab75874 Already tested WB, ms and rat
    FAK antibody ab61113 Already tested WB, ms and rat
    S6K antibody ab47504 Previously tested WB, ms and rat
    PPP1A antibody ab 16476 Previously tested WB, ms and rat
    5HT7 Receptor antibody ab13898 WB and mouse already tested.
    NMDAR1 antibody ab28669 Previously tested WB, ms and rat
    Dopamine Receptor D1 antibody ab85608 Previously tested WB, ms and rat
    Glutamate Receptor 1 (AMPA subtype) antibody ab31232 Previously tested WB and rat
    Nicotinic Acetylcholine Receptor alpha 4 antibody ab41172 Previously tested WB and mouse
    Nicotinic Acetylcholine Receptor alpha 7 antibody ab23832 Previously tested WB and rat

    For the antibodies we can provide a discount for, I can offer a discount off a future purchase if you buy the antibody(ies) now, test them in mouse, rat or both as indicated above, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount provided for each individual antibody would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test these antibodies in mouse, rat or both. I will then send a discount code. This code must be issued before purchasing the antibodies so please wait for my reply before ordering.

    2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).

    3. Test it in mouse, rat or both.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN] ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for the species, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Also, we are able to provide a discount for any order of 5 items or more. Therefore, once you have decided which antibodies you are going to purchase, please contact our customer service department before you place the order with a full list of the antibodies you wish to buy (orders@abcam.com). They will then be able to provide you with a quote.

    I hope this will be helpful to you. I look forward to receiving confirmation that you would like to receive the discount codes. If you have any further questions, please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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