Anti-CAMKK2 antibody (ab96531)
Key features and details
- Rabbit polyclonal to CAMKK2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CAMKK2 antibody
See all CAMKK2 primary antibodies -
Description
Rabbit polyclonal to CAMKK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 1-15 of CAMKK2 isoform 5 (NP_705720)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab96531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 55 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1. -
Tissue specificity
Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10645 Human
- Omim: 615002 Human
- SwissProt: Q96RR4 Human
- Unigene: 297343 Human
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Alternative names
- Calcium/calmodulin dependent protein kinase beta antibody
- Calcium/calmodulin dependent protein kinase kinase 2 antibody
- Calcium/calmodulin dependent protein kinase kinase 2 beta antibody
see all
Images
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Western blot - Anti-CAMKK2 antibody (ab96531)Anti-CAMKK2 antibody (ab96531) at 1/500 dilution (10% SDS PAGE) + H1299 whole cell lysate at 30 µg
Predicted band size: 55 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CAMKK2 antibody (ab96531)Immunofluorescence analysis of paraformaldehyde fixed HeLa using ab96531 at 1/200 dilution: (1) without DNA probe; (2) merged with DNA probe.
Protocols
References (3)
ab96531 has been referenced in 3 publications.
- Cao C et al. A Comparison of Gastric and Jejunal Feeding in Hypercatabolism Associated With Hypothalamic AMPK-Autophagy-POMC in Endotoxemic Rats. JPEN J Parenter Enteral Nutr 44:481-490 (2020). PubMed: 31148205
- Yang J et al. Store-operated calcium entry-activated autophagy protects EPC proliferation via the CAMKK2-MTOR pathway in ox-LDL exposure. Autophagy 13:82-98 (2017). PubMed: 27791458
- Liu DM et al. CAMKK2, Regulated by Promoter Methylation, is a Prognostic Marker in Diffuse Gliomas. CNS Neurosci Ther 22:518-24 (2016). PubMed: 27012733
