Anti-CAMKK2 antibody (ab96531)

Reviews (1)Q&A (2)References (3)
Western blot - Anti-CAMKK2 antibody (ab96531)
  • Immunocytochemistry/ Immunofluorescence - Anti-CAMKK2 antibody (ab96531)

Key features and details

  • Rabbit polyclonal to CAMKK2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CAMKK2 antibody
    See all CAMKK2 primary antibodies

  • Description

    Rabbit polyclonal to CAMKK2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-15 of CAMKK2 isoform 5 (NP_705720)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab96531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.

  • Tissue specificity

    Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.

  • Domain

    The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
    The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.

  • Post-translational
    modifications

    Autophosphorylated.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Calcium/calmodulin dependent protein kinase beta antibody
      • Calcium/calmodulin dependent protein kinase kinase 2 antibody
      • Calcium/calmodulin dependent protein kinase kinase 2 beta antibody
      • Calcium/calmodulin dependent protein kinase kinase beta antibody
      • Calcium/calmodulin-dependent protein kinase kinase 2 antibody
      • Calcium/calmodulin-dependent protein kinase kinase beta antibody
      • CaM kinase kinase beta antibody
      • CaM KK beta antibody
      • CaM-kinase kinase 2 antibody
      • CaM-kinase kinase beta antibody
      • CaM-KK 2 antibody
      • CaM-KK beta antibody
      • CaMKK 2 antibody
      • CAMKK antibody
      • CaMKK beta antibody
      • CAMKK beta protein antibody
      • Camkk2 antibody
      • CAMKKB antibody
      • KKCC2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-CAMKK2 antibody (ab96531)
      Western blot - Anti-CAMKK2 antibody (ab96531)
      Anti-CAMKK2 antibody (ab96531) at 1/500 dilution (10% SDS PAGE) + H1299 whole cell lysate at 30 µg

      Predicted band size: 55 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CAMKK2 antibody (ab96531)
      Immunocytochemistry/ Immunofluorescence - Anti-CAMKK2 antibody (ab96531)
      Immunofluorescence analysis of paraformaldehyde fixed HeLa using ab96531 at 1/200 dilution: (1) without DNA probe; (2) merged with DNA probe.

    References (3)

    Publishing research using ab96531? Please let us know so that we can cite the reference in this datasheet.

    ab96531 has been referenced in 3 publications.

    • Cao C  et al. A Comparison of Gastric and Jejunal Feeding in Hypercatabolism Associated With Hypothalamic AMPK-Autophagy-POMC in Endotoxemic Rats. JPEN J Parenter Enteral Nutr 44:481-490 (2020). PubMed: 31148205
    • Yang J  et al. Store-operated calcium entry-activated autophagy protects EPC proliferation via the CAMKK2-MTOR pathway in ox-LDL exposure. Autophagy 13:82-98 (2017). PubMed: 27791458
    • Liu DM  et al. CAMKK2, Regulated by Promoter Methylation, is a Prognostic Marker in Diffuse Gliomas. CNS Neurosci Ther 22:518-24 (2016). PubMed: 27012733

    Customer reviews and Q&As

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    1-2 of 2 Q&A

    Question

    I have a customer enquiry about antibodies to CAMKK and LKB1, application = WB / IF, species = dictyostelium

    I know that you do not have antibodies to these targets tested in Dictyostelium, but I had trouble finding out about identity of the proteins between the target species and Dicty. So I am hoping you can get some information in a more expedient fashion that I can pass onto the customer.

    I guess it might be possible that at Trial (testing) discount might be applicable?

    Read More
    Answer

    Thank you for contacting us.

    I have performed a sequence alignment for LKB1 for Dictyostelium and for human (which is used to raise most of our antibodies.) Unfortunately there is only a total of 33% homology between the two proteins. I have contacted the lab and indeed all the immunogens of the anti-LKB1antibodies guaranteed to work in WB/IF in our catalogue have a 28-50% sequence homology with the Dictyostelium protein and therefore are not likely to work.

    The amino acid sequence for LKB1 for Dictyostelium that I used can be found at http://www.uniprot.org/uniprot/Q15831.fasta. Unfortunately, this is the predicted amino acid sequence for the species and has not been confirmed therefore, if your customer has the verified sequence of this protein, then I will be happy to repeat these alignments to verify the homology.

    Regarding an anti-CAMKK antibody, I am not able to recommend a suitable one unless I have the Dictyostelium amino acid sequence for this protein. If your customer could send it, then again I will be happy to perform the sequence alignments.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I have a customer enquiry about antibodies to CAMKK and LKB1, application = WB / IF, species = dictyostelium

    I know that you do not have antibodies to these targets tested in Dictyostelium, but I had trouble finding out about identity of the proteins between the target species and Dicty. So I am hoping you can get some information in a more expedient fashion that I can pass onto the customer.

    I guess it might be possible that at Trial (testing) discount might be applicable?

    I look forward to your feedback which I can forward to the customer.

    Read More
    Answer

    Thank you for contacting us.

    I have found the amino acid sequence for LKB1 for Dictyostelium which can be found at http://www.uniprot.org/uniprot/Q15831.fasta

    I have contacted the lab to see if any of our antibodies would be compatible for use in Dictyostelium.

    I have not been able to find the sequence for CAMKK for Dictyostelium. Could you ask your customer to send it if they have it so that I may perform the sequence alignments?

    I look forward to your reply.

    Read More

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