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I have a customer enquiry about antibodies to CAMKK and LKB1, application = WB / IF, species = dictyostelium
I know that you do not have antibodies to these targets tested in Dictyostelium, but I had trouble finding out about identity of the proteins between the target species and Dicty. So I am hoping you can get some information in a more expedient fashion that I can pass onto the customer.
I guess it might be possible that at Trial (testing) discount might be applicable?
Asked on Dec 13 2012
Thank you for contacting us.
I have performed a sequence alignment for LKB1 for Dictyostelium and for human (which is used to raise most of our antibodies.) Unfortunately there is only a total of 33% homology between the two proteins. I have contacted the lab and indeed all the immunogens of the anti-LKB1antibodies guaranteed to work in WB/IF in our catalogue have a 28-50% sequence homology with the Dictyostelium protein and therefore are not likely to work.
The amino acid sequence for LKB1 for Dictyostelium that I used can be found at http://www.uniprot.org/uniprot/Q15831.fasta. Unfortunately, this is the predicted amino acid sequence for the species and has not been confirmed therefore, if your customer has the verified sequence of this protein, then I will be happy to repeat these alignments to verify the homology.
Regarding an anti-CAMKK antibody, I am not able to recommend a suitable one unless I have the Dictyostelium amino acid sequence for this protein. If your customer could send it, then again I will be happy to perform the sequence alignments.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Dec 13 2012