Anti-CAMLG/CAML antibody (ab236655)
Key features and details
- Rabbit polyclonal to CAMLG/CAML
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CAMLG/CAML antibody
See all CAMLG/CAML primary antibodies -
Description
Rabbit polyclonal to CAMLG/CAML -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CAMLG/CAML aa 1-200.
Database link: P49069 -
Positive control
- WB: Jurkat, HEK-293, U-87 MG and SH-SY5Y whole cell lysates. IHC-P: HepG2 cells. IP: HEK-293 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 33 kDa.
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IP |
1/200 - 1/2000.
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ICC/IF |
1/50 - 1/500.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 33 kDa. |
IP
1/200 - 1/2000. |
ICC/IF
1/50 - 1/500. |
Target
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Relevance
The immunosuppressant drug cyclosporin A blocks a calcium dependent signal from the T cell receptor (TCR) that normally leads to T cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin. [Entrez Gene] -
Cellular localization
Membrane; Multi pass membrane protein. -
Database links
- Entrez Gene: 819 Human
- Omim: 601118 Human
- SwissProt: P49069 Human
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Alternative names
- calcium modulating cyclophilin ligand antibody
- calcium modulating ligand antibody
- Calcium signal modulating cyclophilin ligand antibody
see all
Images
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All lanes : Anti-CAMLG/CAML antibody (ab236655) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 33 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CAMLG/CAML (green) using ab236655 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
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CAMLG/CAML was immunoprecipitated from 500 μg HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract with ab236655 at 1/200 dilution.
Lane 1: Control rabbit IgG (1 μg) IP in HEK-293 whole cell lysate.
Lane 2: ab236655 IP in HEK-293 whole cell lysate.
Lane 3: HEK-293 whole cell lysate (10 μg) Input.
HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236655 has not yet been referenced specifically in any publications.