Key features and details
- Rabbit polyclonal to CAMLG/CAML
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CAMLG/CAML antibody
See all CAMLG/CAML primary antibodies
DescriptionRabbit polyclonal to CAMLG/CAML
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CAMLG/CAML aa 1-189.
MESMAVATDGGERPGVPAGSGLSASQRRAELRRRKLLMNSEQRINRIMGF HRPGSGAEEESQTKSKQQDSDKLNSLSVPSVSKRVVLGDSVSTGTTDQQG GVAEVKGTQLGDKLDSFIKPPECSSDVNLELRQRNRGDLTADSVQRGSRH GLEQYLSRFEEAMKLRKQLISEKPSQEDGNTTEEFDSFR
Database link: P49069
- WB: Jurkat, HEK-293, U-87 MG and SH-SY5Y whole cell lysates. IHC-P: HepG2 cells. IP: HEK-293 whole cell lysate.
This product was previously labelled as CAMLG
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 33 kDa.|
|IP||1/200 - 1/2000.|
|ICC/IF||1/50 - 1/500.|
RelevanceThe immunosuppressant drug cyclosporin A blocks a calcium dependent signal from the T cell receptor (TCR) that normally leads to T cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin. [Entrez Gene]
Cellular localizationMembrane; Multi pass membrane protein.
- calcium modulating cyclophilin ligand antibody
- calcium modulating ligand antibody
- Calcium signal modulating cyclophilin ligand antibody
All lanes : Anti-CAMLG/CAML antibody (ab236655) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 33 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CAMLG/CAML (green) using ab236655 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
CAMLG/CAML was immunoprecipitated from 500 μg HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract with ab236655 at 1/200 dilution.
Lane 1: Control rabbit IgG (1 μg) IP in HEK-293 whole cell lysate.
Lane 2: ab236655 IP in HEK-293 whole cell lysate.
Lane 3: HEK-293 whole cell lysate (10 μg) Input.
HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236655 has not yet been referenced specifically in any publications.