Overview

  • Product name
  • Description
    Sheep polyclonal to cAMP
  • Host species
    Sheep
  • Specificity
    This antibody reacts with cyclic AMP and to lesser degrees with other 5’ nucleotides.
  • Tested applications
    Suitable for: ELISA, RIA, Othermore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Succinylated cAMP conjugated to bovine serum albumin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
This antibody may be used in radioimmunoassays to detect and quantitate cAMP; 50% binding is achieved with a 1:1,000 dilution.

 

Other Use at an assay dependent concentration.

Target

  • Relevance
    Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.
  • Cellular localization
    Secreted
  • Alternative names
    • 3' 5' cyclic adenosine monophosphate antibody
    • Cyclic adenosine monophosphate antibody
    • Cyclic AMP antibody

References

This product has been referenced in:
  • Sweeny L  et al. Evaluation of tyrosine receptor kinases in the interactions of head and neck squamous cell carcinoma cells and fibroblasts. Oral Oncol 48:1242-9 (2012). Read more (PubMed: 22795534) »
  • Parro V  et al. A microbial oasis in the hypersaline Atacama subsurface discovered by a life detector chip: implications for the search for life on Mars. Astrobiology 11:969-96 (2011). Read more (PubMed: 22149750) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

ab832 may cross-react with other 5'-nucleotides, however sufficient dilution can eliminate this cross-reactivity while retaining reactivity with cAMP. Working dilution should be optimized by the end user.

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Answer

Thank you for your enquiry. I have received replies from the four product originators. Here is the information that you requested for: ab12414 - First lots were sold in 1987 so before 2003. ab832 - Definitely before 2003. ab2345 - Not certain but would say after 2003. ab24851 - After 2003. Please don't hesitate to contact us again if you have further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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