Recombinant
RabMAb

Recombinant Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)

Overview

  • Product name

    Anti-cAMP antibody [EP8471] - BSA and Azide free
    See all cAMP primary antibodies
  • Description

    Rabbit monoclonal [EP8471] to cAMP - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody does not cross react with cGMP, GMP, GDP, cIMP, cCMP, or adenosine.
  • Tested applications

    Suitable for: ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to cAMP conjugated to keyhole limpet haemocyanin.

  • General notes

    Ab179459 is the carrier-free version of ab134901. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab179459 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP8471
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.
  • Cellular localization

    Secreted
  • Alternative names

    • 3' 5' cyclic adenosine monophosphate antibody
    • Cyclic adenosine monophosphate antibody
    • Cyclic AMP antibody

Images

  • Competitive ELISA assay using ab134901. 50 µl cAMP-SPDP-BSA was coated in 96-wells. After 1% BSA blocking, serial dilution of ab134901, 25 µl of cAMP, and H2O (negative control) were added. HRP conjugated goat anti-rabbit IgG-Fc antibody was used to develop the color.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134901).

  • ab134901 staining cAMP in SK-N-SH cells treated with (R)-(+)-Methanandamide (ethanol solution) (ab120361), by ICC/IF. Increase in cAMP expression correlates with increased concentration of (R)-(+)-Methanandamide (ethanol solution), as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120361 ((R)-(+)-Methanandamide (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab134901 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134901).

  • This ICC/IF data was generated using the same anti-cAMP antibody clone, EP8471, in a different buffer formulation (cat# ab134901).

    ab134901 staining cAMP in SK-N-SH cells treated with (R)-(+)-Methanandamide (ethanol solution) (ab120361), by ICC/IF. Increase in cAMP expression correlates with increased concentration of (R)-(+)-Methanandamide (ethanol solution), as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120361 ((R)-(+)-Methanandamide (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab134901 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

  • This ELISA data was generated using the same anti-cAMP antibody clone, EP8471, in a different buffer formulation (cat# ab134901).

    Competitive inhibition ELISA using ab134901. 50µl of cAMP-SPDP-BSA was coated in 96-wells. After 1% BSA blocking, 25 µl cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, H2O and 25 µl of ab134901 were added. HRP conjugated goat anti-rabbit IgG-Fc-HR antibody was used to develop the color.

References

ab179459 has not yet been referenced specifically in any publications.

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