Product namecAMP Assay Kit (Competitive ELISA)
See all cAMP kits
Sample typeUrine, Serum, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
Sensitivity> 0.02 µM
Range0.02 µM - 2 µM
Assay time3h 30m
Species reactivityReacts with: Mammals, Other species
cAMP Assay Kit ab65355 uses a sensitive colorimetric competitive ELISA method to quantify cAMP (cyclic AMP) levels in samples from mammals and other species. It has a sensitivity of 0.02 µM.
The cAMP assay protocol uses a 96-well plate supplied coated with Protein G. The Protein G binds an anti-cAMP antibody which is added to the plate.
HRP-labeled cAMP, and free cAMP within a sample, are then added to each well of the plate. The cAMP assay is based on the competition between the HRP-labeled cAMP and the free cAMP for the fixed number of cAMP antibody binding sites on the plate.
The more free cAMP is present, the less HRP-labeled cAMP is bound. After a washing step, the amount of bound HRP-labeled cAMP is detected using a colorimetric HRP substrate measured by absorbance at OD 450 nm. The level of signal is compared to a standard curve for free cAMP, which is run at the same time as the samples.
This kit uses an acetylation step to achieve the maximum sensitivity.
cAMP assay protocol summary:
- add prepared samples and standards to tubes
- add neutralizing buffer
- add acetylating reagent mix, vortex and incubate for 10 min at room temp
- add assay buffer
- transfer to protein-G 96-well plate
- add cAMP antibody and incubate for 1 hr at room temp
- add cAMP-HRP and incubate for 1 hr at room temp
- wash with assay buffer
- add HRP developer and incubate for 1 hr at room temp
- stop reaction with HCl
- analyze with microplate reader
This kit was previously called cAMP Assay Kit - Direct Immunoassay.
If you are using fluorometric detection, we recommend cAMP Assay Kit (Fluorometric) (ab138880).
Storage instructionsStore at -20°C. Please refer to protocols.
Storage bufferPreservative: None
Components Identifier 100 tests 10X cAMP Assay Buffer WM 1 x 25ml Acetylating Reagent A Violet 1 x 750µl Acetylating Reagent B Black 1 x 1.5ml cAMP-HRP Green 1 vial HRP Developer Amber 1 x 10ml Neutralizing Buffer NM 1 x 7.5ml Protein G Coated Plate 1 unit Rabbit Anti-cAMP polyclonal Antibody Red 1 vial Standard cAMP (10 nmol) Yellow 1 x 10µl
RelevanceAdenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. It plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals.
- Adenosine 3' 5' cyclic monophosphate
- Adenosine 3’ 5’ cyclic monophosphate
- Cyclic adenosine monophosphate
- Cyclic AMP
- 8-(4-Chlorophenylthio)adenosine 3',5'-cyclic monophosphate (8-CPT-cAMP), Cell permeable cAMP analog. (ab120424)
- Adenosine 3prime,5prime-cyclic monophosphate (cAMP), Second messenger. PKA activator. (ab120493)
- 8-Br-cAMP (8-Bromo-cAMP), PKA activator (ab141448)
- RHC-80267, diacylglycerol lipase inhibitor (ab141758)
- 10kD Spin Column (ab93349)
Concentration of cAMP in response to in vitro haemocyte stimulation with LPS and β-AR (B) agonist/antagonist at 24h. Data presented as mean ± S.D. (N = 6) of cAMP concentration expressed as pmol µL-1. The significant differences among the control and treatedgroups were subjected to one-way ANOVA, followed by Student-Newman-Keuls multiple range test, which revealed two groups (a to b). The concentration of cAMP with the same letter in common do not differ at the P = 0.05 level of significance.
Measurement of cAMP in Jurkat and HepG2 cells with ab65355. Assay performed using the kit protocol.
Standard curve (colorimetric): mean of duplicates (+/-SD)
ab65355 has been referenced in 33 publications.
- Zhao H et al. Hippuric acid and 3-(3-hydroxyphenyl) propionic acid inhibit murine osteoclastogenesis through RANKL-RANK independent pathway. J Cell Physiol 235:599-610 (2020). PubMed: 31271661
- Potnuri AG et al. Involvement of Histamine 2 Receptor in Alpha 1 Adrenoceptor Mediated Cardiac Hypertrophy and Oxidative Stress in H9c2 Cardio Myoblasts. J Cardiovasc Transl Res N/A:N/A (2020). PubMed: 32385805
- Hu Q et al. Oncogenic lncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression. Nat Immunol 20:835-851 (2019). PubMed: 31160797
- Menon A et al. Electrophysiologic and molecular mechanisms of a frameshift NPPA mutation linked with familial atrial fibrillation. J Mol Cell Cardiol 132:24-35 (2019). PubMed: 31077706
- Hurtado Del Pozo C et al. A Receptor of the Immunoglobulin Superfamily Regulates Adaptive Thermogenesis. Cell Rep 28:773-791.e7 (2019). PubMed: 31315054