Overview

  • Product name

    cAMP Assay Kit (Competitive ELISA, Fluorometric)
    See all cAMP kits
  • Detection method

    Fluorescent
  • Sample type

    Urine, Plasma, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Competitive
  • Sensitivity

    1 nM
  • Assay time

    3h 00m
  • Product overview

    cAMP Assay Kit ab138880 uses a sensitive fluorometric competitive ELISA method to quantify cAMP (cyclic AMP) levels in samples from mammals and other species.


    The cAMP assay protocol uses a 96-well plate supplied coated with an anti-cAMP antibody.


    HRP-labeled cAMP, and free cAMP within a sample, are added to each well of the plate. The cAMP assay is based on the competition between the HRP-labeled cAMP and the free cAMP for the fixed number of cAMP antibody binding sites on the plate.


    The more free cAMP is present, the less HRP-labeled cAMP is bound. After a washing step, the amount of bound HRP-labeled cAMP is detected using a fluorometric HRP substrate. The level of signal is compared to a standard curve for free cAMP, which is run at the same time as the samples.


    Unlike other cAMP assay protocols, this kit does not require an acetylation step to achieve the maximum sensitivity.

  • Notes

    This product was previously called cAMP Direct Immunoassay Detection Kit (Fluorometric).

     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests 10 x 96 tests
    10X Wash Solution 1 x 10ml 1 x 100ml
    AbRed Indicator 1 vial 1 vial
    Anti-cAMP-coated 96-well plate 1 unit 1 x 10 units
    Assay Buffer 1 x 20ml 1 x 100ml
    cAMP Standard 1 x 33µg 1 x 33µg
    Cell Lysis Buffer 1 x 10ml 1 x 100ml
    HRP-cAMP Conjugate 1 vial 1 vial
    Hydrogen Peroxide Solution 1 x 50µl 1 x 250µl
    Substrate Buffer 1 x 10ml 1 x 100ml
  • Research areas

  • Relevance

    Adenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. It plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals.
  • Alternative names

    • Adenosine 3' 5' cyclic monophosphate
    • Adenosine 3’ 5’ cyclic monophosphate
    • Cyclic adenosine monophosphate
    • Cyclic AMP

Images

  • cAMP dose response was measured with ab138880 in a solid black 96-well plate with a fluorescence microplate reader. The kit can detect as low as 1 nM cAMP in a 100 µl reaction volume.
  • cAMP dose response in various amounts of HeLa cells after and without forskolin treatment (100 micromolar - ab120058)

  • cAMP dose response in various amounts of 3T3-L1 cells after and without forskolin treatment (100 micromolar - ab120058)

  • cAMP concentration (nM) measured in various biological fluids following assay protocol.

Protocols

References

This product has been referenced in:

  • Bhattarai Y  et al. Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion. Cell Host Microbe 23:775-785.e5 (2018). Read more (PubMed: 29902441) »
  • Tian S  et al. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. PLoS Biol 16:e2006951 (2018). Read more (PubMed: 30481169) »
See all 3 Publications for this product

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