Key features and details
- Sensitivity: 0.94 ng/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Species independent
Product namecAMP ELISA Kit
See all cAMP kits
Sample typeSerum, Plasma, Other biological fluids
Range1.56 ng/ml - 100 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Species independent
cAMP ELISA kit (ab285319, E4715) uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.
The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsPlease refer to protocols.
Components 96 tests 100X Biotinylated Detection Antibody 1 x 120µl 100X HRP-Conjugate 1 x 120µl 25X Wash buffer 1 x 30ml Biotinylated Detection Antibody Diluent 1 x 14ml HRP-Conjugate Diluent 1 x 14ml Micro ELISA Plate 1 unit Plate Sealer 5 units Standard 2 vials Standard and Sample Diluent 1 x 20ml Stop Solution 1 x 10ml Substrate Reagent 1 x 10ml
Relevancedblinks not applicable
- 3'-5'-cyclic adenosine monophosphate
- Cyclic adenosine monophosphate
- cyclic AMP
ab285319 has not yet been referenced specifically in any publications.