Overview

  • Product name
    Anti-Campylobacter jejuni antibody [BGN/2E10]
    See all Campylobacter jejuni primary antibodies
  • Description
    Mouse monoclonal [BGN/2E10] to Campylobacter jejuni
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with Campylobacter jejuni. Not yet tested in other species.
  • Immunogen

    Native, Type 1 Campylobacter jejuni

  • General notes


    ab 54125 reacts with a soluble excreted antigen in EIA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require fresh cultured organisms.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Purified IgM
  • Purification notes
    ab54125 was purified by Thiosorb M chromatography
  • Primary antibody notes
    ab 54125 reacts with a soluble excreted antigen in EIA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require fresh cultured organisms.
  • Clonality
    Monoclonal
  • Clone number
    BGN/2E10
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 25401947

Target

  • Relevance
    Campylobacteriosis is an infectious disease caused by bacteria of the genus Campylobacter. Most people who become ill with campylobacteriosis get diarrhea, cramping, abdominal pain, and fever within 2 to 5 days after exposure to the organism. The diarrhea may be bloody and can be accompanied by nausea and vomiting. The illness typically lasts 1 week. Some persons who are infected with Campylobacter don't have any symptoms at all. In persons with compromised immune systems, Campylobacter occasionally spreads to the bloodstream and causes a serious life threatening infection. The Campylobacter organism is actually a group of spiral shaped bacteria that can cause disease in humans and animals. Most human illness is caused by one species, called Campylobacter jejuni, but 1% of human Campylobacter cases are caused by other species.
  • Cellular localization
    whole cell

References

This product has been referenced in:
  • Baily JL  et al. Evidence of land-sea transfer of the zoonotic pathogen Campylobacter to a wildlife marine sentinel species. Mol Ecol 24:208-21 (2015). IHC-P . Read more (PubMed: 25401947) »
  • Haddock G  et al. Campylobacter jejuni 81-176 forms distinct microcolonies on in vitro-infected human small intestinal tissue prior to biofilm formation. Microbiology 156:3079-84 (2010). Read more (PubMed: 20616103) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

This antibody has been used in the following publication:

Haddock G et al. Campylobacter jejuni 81-176 forms distinct microcolonies on in vitro-infected human small intestinal tissue prior to biofilm formation. Microbiology 156:3079-84 (2010).

The protocol used was as follows:

Human ileal sections of 4 μm thickness were mounted onto slides and allowed to dry before placing in a 37 °C oven overnight. Slides were marked with the antibody and date, dewaxed in xylene and rinsed in 100 % alcohol then 95 % alcohol. Slides were then placed in 3 % hydrogen peroxide in methanol for 20 min at room temperature. After washing in water, antigen retrieval was performed by placing the slides in an autoclave for 10 min in citrate buffer, pH 6, at 121 °C. Slides were left to cool to 50 °C before the transfer of slides to a staining chamber. Slides were washed in PBS/Tween-20 twice before applying 25 % normal goat serum for 30 min at room temperature. Antibody against C. jejuni was added to the slides overnight at 4 °C (100 μl, 1 : 600 mouse monoclonal, BGN/2E10, 1 mg ml−1, Abcam ab54125).

You could therefore do a trial run with this antibody at a dilution of 1:600 overnight at 4ºC and then optimise accordingly.

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Answer



ab54125 wurde von uns nur mit Aceton-fixiertem Gewebe getestet und wir haben leider auch sonst keinerlei Anhaltspunkte (Rückmeldung von Kunden, etc.), dass er mit PFA-fixierten Geweben funktioniert.

Da auch der andere Antikörper nicht mit PFA funktioniert hat, schien es nicht per se am Antikörper zu liegen sondern eher daraufhin zu deuten, dass dieses Zielprotein nicht geeignet ist für einen PFA Fixierung.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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