Overview

  • Product name

    Anti-Campylobacter jejuni antibody [BGN/2E10]
    See all Campylobacter jejuni primary antibodies
  • Description

    Mouse monoclonal [BGN/2E10] to Campylobacter jejuni
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Native, Type 1 Campylobacter jejuni

  • General notes


    ab 54125 reacts with a soluble excreted antigen in EIA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require fresh cultured organisms.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Purified IgM
  • Purification notes

    ab54125 was purified by Thiosorb M chromatography
  • Primary antibody notes

    ab 54125 reacts with a soluble excreted antigen in EIA. This determinant is unaffected by frozen storage of specimens, unlike antibodies to flagellar antigens which require fresh cultured organisms.
  • Clonality

    Monoclonal
  • Clone number

    BGN/2E10
  • Isotype

    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 25401947

Target

  • Relevance

    Campylobacteriosis is an infectious disease caused by bacteria of the genus Campylobacter. Most people who become ill with campylobacteriosis get diarrhea, cramping, abdominal pain, and fever within 2 to 5 days after exposure to the organism. The diarrhea may be bloody and can be accompanied by nausea and vomiting. The illness typically lasts 1 week. Some persons who are infected with Campylobacter don't have any symptoms at all. In persons with compromised immune systems, Campylobacter occasionally spreads to the bloodstream and causes a serious life threatening infection. The Campylobacter organism is actually a group of spiral shaped bacteria that can cause disease in humans and animals. Most human illness is caused by one species, called Campylobacter jejuni, but 1% of human Campylobacter cases are caused by other species.
  • Cellular localization

    whole cell

References

This product has been referenced in:

  • Baily JL  et al. Evidence of land-sea transfer of the zoonotic pathogen Campylobacter to a wildlife marine sentinel species. Mol Ecol 24:208-21 (2015). IHC-P . Read more (PubMed: 25401947) »
  • Haddock G  et al. Campylobacter jejuni 81-176 forms distinct microcolonies on in vitro-infected human small intestinal tissue prior to biofilm formation. Microbiology 156:3079-84 (2010). Read more (PubMed: 20616103) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

This antibody has been used in the following publication:

Haddock G et al. Campylobacter jejuni 81-176 forms distinct microcolonies on in vitro-infected human small intestinal tissue prior to biofilm formation. Microbiology 156:3079-84 (2010).

The protocol used was as follows:

Human ileal sections of 4 μm thickness were mounted onto slides and allowed to dry before placing in a 37 °C oven overnight. Slides were marked with the antibody and date, dewaxed in xylene and rinsed in 100 % alcohol then 95 % alcohol. Slides were then placed in 3 % hydrogen peroxide in methanol for 20 min at room temperature. After washing in water, antigen retrieval was performed by placing the slides in an autoclave for 10 min in citrate buffer, pH 6, at 121 °C. Slides were left to cool to 50 °C before the transfer of slides to a staining chamber. Slides were washed in PBS/Tween-20 twice before applying 25 % normal goat serum for 30 min at room temperature. Antibody against C. jejuni was added to the slides overnight at 4 °C (100 μl, 1 : 600 mouse monoclonal, BGN/2E10, 1 mg ml−1, Abcam ab54125).

You could therefore do a trial run with this antibody at a dilution of 1:600 overnight at 4ºC and then optimise accordingly.

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Answer



ab54125 wurde von uns nur mit Aceton-fixiertem Gewebe getestet und wir haben leider auch sonst keinerlei Anhaltspunkte (Rückmeldung von Kunden, etc.), dass er mit PFA-fixierten Geweben funktioniert.

Da auch der andere Antikörper nicht mit PFA funktioniert hat, schien es nicht per se am Antikörper zu liegen sondern eher daraufhin zu deuten, dass dieses Zielprotein nicht geeignet ist für einen PFA Fixierung.

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