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Rabbit polyclonal to CAMSAP1L1
Predicted to work with:
Mouse, Rat, Rabbit, Chicken, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 1439 and 1489 of Human CAMSAP1L1 (EAW91320.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 168 kDa.
Use at 2-5 µg/mg of lysate.
CAMSAP1L1 (Calmodulin regulated spectrin associated protein 1 like protein 1) is related to CAMSAP1. Both contain an N terminal CH (calponin homology domain) which is an actin binding domain found in cytoskeletal and signal transduction proteins. The CAMSAP1 family of proteins also contains a unique C terminal CKK (DUF1781) domain which has been found to bind microtubules. The function of CAMSAP1L1 has not been characterized.
Calmodulin regulated spectrin associated protein 1 like 1 antibody
Calmodulin regulated spectrin associated protein 2 antibody
Western blot - Anti-CAMSAP1L1 antibody (ab86683)
All lanes :
Anti-CAMSAP1L1 antibody (ab86683) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Predicted band size:
Observed band size:
200 kDa (
why is the actual band size different from the predicted?
Additional bands at:
71 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure time of 30 seconds.
Immunoprecipitation - Anti-CAMSAP1L1 antibody (ab86683)
Western Blot detection of CAMSAP1L1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86683 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"