Anti-CAMTA1 antibody (ab64119)

Reviews (1)Q&A (3)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)

    Key features and details

    • Rabbit polyclonal to CAMTA1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-CAMTA1 antibody
      See all CAMTA1 primary antibodies

    • Description

      Rabbit polyclonal to CAMTA1

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Horse, Chicken, Dog

    • Immunogen

      Synthetic peptide conjugated to KLH derived from within residues 1650 to the C-terminus of Human CAMTA1.

      Read Abcam's proprietary immunogen policy (Peptide available as ab66539.)

    • Positive control

      • This antibody gave a positive signal in human hippocampus tissue sections.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab64119 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)
      IHC image of CAMTA1 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64119, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    References (1)

    Publishing research using ab64119? Please let us know so that we can cite the reference in this datasheet.

    ab64119 has been referenced in 1 publication.

    • Lee SJ  et al. Epithelioid hemangioendotheliomas with TFE3 gene translocations are compossible with CAMTA1 gene rearrangements. Oncotarget 7:7480-8 (2016). IHC . PubMed: 26840265

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Question

    We are looking for good antibody for anti-mouse CAMTA antibody. Anti-CAMTA1 antibody (ab64119) is designed to against human Camta1, but its is also predicted to work with Mouse. Could you tell us the seqence of synthetic peptide you used, so we can tell how much difference between human and mouse? Or just tell us if the peptide sequence you used for this antibody is completely conserved in human and mouse. Thanks.

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    Answer

    Thank you for contacting us. The immunogen sequence used to create this product is xxxxxxx. This sequence is completely conserved in mouse. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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    Western blot abreview for Anti-CAMTA1 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (cortex, hippocampus, cerebellum)
    Loading amount
    100 µg
    Specification
    cortex, hippocampus, cerebellum
    Gel Running Conditions
    Non-reduced Denaturing (8% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Abcam user community

    Verified customer

    Submitted Sep 18 2012

    Question

    Thank you for this offer. I will take this offer. But the antibody will be purchased through our lab account. Do you need any proof of purchase?

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    Answer

    I am very pleased to hear you would like to accept our offer and test ab64119 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for WB] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. The active code may be used by your lab and will not be redeemed through your account. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Question

    Thank youvery much for this important information. Could you tell me if you test this antibody by western blot? thanks

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    Answer

    Thank you very much for your interest in ab64119.

    To our knowledge, this has not been tested in Western Blot. Therefore, I can offer a discount off a future purchase if you buy ab64119 now, test it in Western blot and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab64119 inWestern Blot. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

    2. Purchase ab64119 either by phone, fax, or online (www.abcam.com).

    3. Test it in WB.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab64119 turns out to be unsuitable for Western Blot, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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