Anti-CAMTA1 antibody (ab64119)

Reviews (1)Q&A (3)References (1)

Overview

  • Product name

    Anti-CAMTA1 antibody
    See all CAMTA1 primary antibodies

  • Description

    Rabbit polyclonal to CAMTA1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Horse, Chicken, Dog

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1650 to the C-terminus of Human CAMTA1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab66539.)

  • Positive control

    • This antibody gave a positive signal in human hippocampus tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab64119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)
    IHC image of CAMTA1 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64119, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References

This product has been referenced in:

  • Lee SJ  et al. Epithelioid hemangioendotheliomas with TFE3 gene translocations are compossible with CAMTA1 gene rearrangements. Oncotarget 7:7480-8 (2016). IHC . Read more (PubMed: 26840265) »
See 1 Publication for this product

Publishing research using ab64119? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

We are looking for good antibody for anti-mouse CAMTA antibody. Anti-CAMTA1 antibody (ab64119) is designed to against human Camta1, but its is also predicted to work with Mouse. Could you tell us the seqence of synthetic peptide you used, so we can tell how much difference between human and mouse? Or just tell us if the peptide sequence you used for this antibody is completely conserved in human and mouse. Thanks.

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Answer

Thank you for contacting us. The immunogen sequence used to create this product is xxxxxxx. This sequence is completely conserved in mouse. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Western blot abreview for Anti-CAMTA1 antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (cortex, hippocampus, cerebellum)
Loading amount
100 µg
Specification
cortex, hippocampus, cerebellum
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Sep 18 2012

Question

Thank you for this offer. I will take this offer. But the antibody will be purchased through our lab account. Do you need any proof of purchase?

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Answer

I am very pleased to hear you would like to accept our offer and test ab64119 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for WB] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. The active code may be used by your lab and will not be redeemed through your account. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

Thank youvery much for this important information. Could you tell me if you test this antibody by western blot? thanks

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Answer

Thank you very much for your interest in ab64119.

To our knowledge, this has not been tested in Western Blot. Therefore, I can offer a discount off a future purchase if you buy ab64119 now, test it in Western blot and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab64119 inWestern Blot. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase ab64119 either by phone, fax, or online (www.abcam.com).

3. Test it in WB.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab64119 turns out to be unsuitable for Western Blot, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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