Overview

  • Product name
    Anti-CAMTA1 antibody
    See all CAMTA1 primary antibodies
  • Description
    Rabbit polyclonal to CAMTA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity

    Predicted to work with: Mouse, Horse, Chicken, Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1650 to the C-terminus of Human CAMTA1.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab66539.)

  • Positive control
    • This antibody gave a positive signal in human hippocampus tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab64119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)
    IHC image of CAMTA1 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64119, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References

This product has been referenced in:
  • Lee SJ  et al. Epithelioid hemangioendotheliomas with TFE3 gene translocations are compossible with CAMTA1 gene rearrangements. Oncotarget 7:7480-8 (2016). IHC . Read more (PubMed: 26840265) »

See 1 Publication for this product

Publishing research using ab64119? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

10
Votes: Highest First

We are looking for good antibody for anti-mouse CAMTA antibody. Anti-CAMTA1 antibody (ab64119) is designed to against human Camta1, but its is also predicted to work with Mouse. Could you tell us the seqence of synthetic peptide you used, so we can t...

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Thank you for contacting us. The immunogen sequence used to create this product is xxxxxxx. This sequence is completely conserved in mouse. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advic...

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Western blot abreview for Anti-CAMTA1 antibody

 Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (cortex, hippocampus, cerebellum)
Loading amount
100 µg
Specification
cortex, hippocampus, cerebellum
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Sep 18 2012

Thank you for this offer. I will take this offer. But the antibody will be purchased through our lab account. Do you need any proof of purchase?

I am very pleased to hear you would like to accept our offer and test ab64119 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for WB] and include this code in the &ldqu...

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Thank youvery much for this important information. Could you tell me if you test this antibody by western blot? thanks

Thank you very much for your interest in ab64119.

To our knowledge, this has not been tested in Western Blot. Therefore, I can offer a discount off a future purchase if you buy ab64119 now, test it in Western blot and submit feedback to us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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