PD-1 and PD-L1 pathway research solutions

Targeting the PD-1/PD-L1 axis has been a successful therapeutic strategy against several tumor types. Learn more about our highly validated reagents that can help you explore this key pathway.​

PD-1 and PD-L1 SimpleStep ELISA® kits

SimpleStep ELISA kits are one-step assays for quantification of your target protein​.

  • Validated and developed in relevant biological matrices - it will work with your sample
  • Proprietary tag instead of biotin-streptavidin to eliminate interference –compatible with cell culture supernatant
  • Optimized buffers to improve sensitivity and reduce non-specific antibody binding

Assay TimeSpeciesDetectionSensitivityRange
PD-1 90minMouseColorimetric21 pg/mL125–8,000 pg/mL
PD-L1 [28-8]90minHumanColorimetric3.81 pg/mL21.9–1,400 pg/mL

The panel above shows interpolated concentrations of native PD1 in mouse EL-4 cell extract samples based on  a 180  µg/mL extract load (left). Interpolated concentrations of native PD-L1 in human Jurkat stimulated with LPS and IFN-gamma, placenta, and thyroid based on a 1,000 µg/mL extract load (right). 

Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

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