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Targeting the PD-1/PD-L1 axis has been a successful therapeutic strategy against several tumor types. The following table outlines tools to study the PD-1 and PD-L1 pathway:
PD-1 | PD-L1 | |
Antibodies | ||
Conjugated antibodies | ||
PBS only formulations | BSA & Azide free | BSA & Azide free |
ELISA kits | Mouse PD-1 SimpleStep ELISA® kit | Human PD-L1 [28-8] SimpleStep ELISA® kit |
Recombinant proteins |
SimpleStep ELISA kits are one-step assays for quantification of your target protein.
Assay Time | Species | Detection | Sensitivity | Range | |
PD-1 | 90min | Mouse | Colorimetric | 21 pg/mL | 125–8,000 pg/mL |
PD-L1 [28-8] | 90min | Human | Colorimetric | 3.81 pg/mL | 21.9–1,400 pg/mL |
The panel above shows interpolated concentrations of native PD1 in mouse EL-4 cell extract samples based on a 180 µg/mL extract load (left). Interpolated concentrations of native PD-L1 in human Jurkat stimulated with LPS and IFN-gamma, placenta, and thyroid based on a 1,000 µg/mL extract load (right).
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.