Key features and details
- Rabbit polyclonal to CAND1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CAND1 antibody
See all CAND1 primary antibodies
DescriptionRabbit polyclonal to CAND1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to Human CAND1 aa 1180-1230 (internal sequence).
Database link: NP_060918.2
- 293T, NIH3T3 and HeLa cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 136 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEnhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins.
Sequence similaritiesBelongs to the CAND family.
Contains 27 HEAT repeats.
- Information by UniProt
- CAND 1 antibody
- Cand1 antibody
- CAND1_HUMAN antibody
All lanes : Anti-CAND1 antibody (ab101987) at 0.1 µg
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Predicted band size: 136 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse hybridoma tumor (right) tissues labelling CAND1 with ab101987 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
Detection of CAND1 by Western blot of Immunprecipitate.
ab101987, at 1 µg/ml, staining CAND1 in HeLa whole cell lysate immunoprecipitated using ab101987 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
ab101987 has not yet been referenced specifically in any publications.