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    cand1-antibody-ab101987.pdf

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Epigenetics and Nuclear Signaling Cell cycle Ubiquitin ligases
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Anti-CAND1 antibody (ab101987)

  • Datasheet
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Western blot - Anti-CAND1 antibody (ab101987)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody (ab101987)
  • Immunoprecipitation - Anti-CAND1 antibody (ab101987)

Key features and details

  • Rabbit polyclonal to CAND1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-CAND1 antibody
    See all CAND1 primary antibodies
  • Description

    Rabbit polyclonal to CAND1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human CAND1 aa 1180-1230 (internal sequence).
    Database link: NP_060918.2

  • Positive control

    • 293T, NIH3T3 and HeLa cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • NIH/3T3 nuclear extract lysate (ab14874)
    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)

Applications

Our Abpromise guarantee covers the use of ab101987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Enhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins.
  • Sequence similarities

    Belongs to the CAND family.
    Contains 27 HEAT repeats.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q86VP6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 452060 Chimpanzee
    • Entrez Gene: 538086 Cow
    • Entrez Gene: 55832 Human
    • Entrez Gene: 71902 Mouse
    • Entrez Gene: 100173509 Orangutan
    • Entrez Gene: 117152 Rat
    • Omim: 607727 Human
    • SwissProt: A7MBJ5 Cow
    • SwissProt: Q86VP6 Human
    • SwissProt: Q6ZQ38 Mouse
    • SwissProt: Q5R6L5 Orangutan
    • SwissProt: P97536 Rat
    • Unigene: 546407 Human
    • Unigene: 203965 Mouse
    • Unigene: 32934 Rat
    see all
  • Alternative names

    • CAND 1 antibody
    • Cand1 antibody
    • CAND1_HUMAN antibody
    • Cullin associated and neddylation dissociated 1 antibody
    • Cullin associated and neddylation dissociated protein 1 antibody
    • Cullin associated NEDD8 dissociated protein 1 antibody
    • Cullin-associated and neddylation-dissociated protein 1 antibody
    • Cullin-associated NEDD8-dissociated protein 1 antibody
    • DKFZP434M1414 antibody
    • FLJ10114 antibody
    • FLJ10929 antibody
    • FLJ38691 antibody
    • FLJ90441 antibody
    • KIAA0829 antibody
    • p120 CAND1 antibody
    • TATA binding protein interacting protein 120A antibody
    • TBP interacting protein antibody
    • TBP interacting protein of 120 kDa A antibody
    • TBP Interacting Protein TIP120A antibody
    • TBP-interacting protein 120A antibody
    • TBP-interacting protein of 120 kDa A antibody
    • TIP120 antibody
    • TIP120 protein antibody
    • TIP120A antibody
    see all

Images

  • Western blot - Anti-CAND1 antibody (ab101987)
    Western blot - Anti-CAND1 antibody (ab101987)
    All lanes : Anti-CAND1 antibody (ab101987) at 0.1 µg

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg

    Predicted band size: 136 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody (ab101987)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody (ab101987)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse hybridoma tumor (right) tissues labelling CAND1 with ab101987 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.

  • Immunoprecipitation - Anti-CAND1 antibody (ab101987)
    Immunoprecipitation - Anti-CAND1 antibody (ab101987)

    Detection of CAND1 by Western blot of Immunprecipitate.
    ab101987, at 1 µg/ml, staining CAND1 in HeLa whole cell lysate immunoprecipitated using ab101987 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab101987? Please let us know so that we can cite the reference in this datasheet.

    ab101987 has not yet been referenced specifically in any publications.

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