Anti-CAND1 antibody (ab101987)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CAND1 antibody
See all CAND1 primary antibodies -
Description
Rabbit polyclonal to CAND1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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Immunogen
Synthetic peptide corresponding to Human CAND1 aa 1180-1230 (internal sequence).
Database link: NP_060918.2 -
Positive control
- 293T, NIH3T3 and HeLa cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab101987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 136 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Enhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins. -
Sequence similarities
Belongs to the CAND family.
Contains 27 HEAT repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 452060 Chimpanzee
- Entrez Gene: 538086 Cow
- Entrez Gene: 55832 Human
- Entrez Gene: 71902 Mouse
- Entrez Gene: 100173509 Orangutan
- Entrez Gene: 117152 Rat
- Omim: 607727 Human
- SwissProt: A7MBJ5 Cow
see all -
Alternative names
- CAND 1 antibody
- Cand1 antibody
- CAND1_HUMAN antibody
see all
Images
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Western blot - Anti-CAND1 antibody (ab101987)All lanes : Anti-CAND1 antibody (ab101987) at 0.1 µg
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Predicted band size: 136 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody (ab101987)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse hybridoma tumor (right) tissues labelling CAND1 with ab101987 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-CAND1 antibody (ab101987)Detection of CAND1 by Western blot of Immunprecipitate.
ab101987, at 1 µg/ml, staining CAND1 in HeLa whole cell lysate immunoprecipitated using ab101987 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
References
ab101987 has not yet been referenced specifically in any publications.
