Product nameAnti-CAND1 antibody
See all CAND1 primary antibodies
DescriptionRabbit polyclonal to CAND1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human kidney tissue, RT-4, U-251 MG and tonsil lysates. SiHa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/250. Predicted molecular weight: 136 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.|
FunctionEnhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins.
Sequence similaritiesBelongs to the CAND family.
Contains 27 HEAT repeats.
- Information by UniProt
- CAND 1 antibody
- Cand1 antibody
- CAND1_HUMAN antibody
All lanes : Anti-CAND1 antibody (ab204554) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 136 kDa
Immunofluorescence analysis of SiHa cells labeling CAND1 with ab204554 at 4 µg/mL.
Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CAND1 with ab204554 at 1/1000 dilution.
ab204554 has not yet been referenced specifically in any publications.