Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14242(B)] to CAND1 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-CAND1 antibody [EPR14242(B)] - C-terminal
See all CAND1 primary antibodies
DescriptionRabbit monoclonal [EPR14242(B)] to CAND1 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
within Human CAND1 aa 1200 to the C-terminus. The exact sequence is proprietary.
Database link: Q86VP6
- Jurkat cells, HeLa cells, Human prostatic hyperplasia tissue, Saos 2 cell line lysate, 293T cell line lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 136 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
|IP||1/40 - 1/60.|
FunctionEnhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins.
Sequence similaritiesBelongs to the CAND family.
Contains 27 HEAT repeats.
- Information by UniProt
- CAND 1 antibody
- Cand1 antibody
- CAND1_HUMAN antibody
Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling CAND1 with ab181216 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution and counterstained with Dapi (blue).
All lanes : Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216) at 1/50000 dilution
Lane 1 : 293T cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : Jurkat cell line lysate
Lane 4 : Saos 2 cell line lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 136 kDa
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling CAND1 with ab181216 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot analysis of immunoprecipitation pellet from Hela cell lysate immunoprecipitated using ab181216 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling CAND1 with ab181216 at 1/20 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
ab181216 has not yet been referenced specifically in any publications.