Recombinant
RabMAb

Recombinant Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216)

Overview

  • Product name

    Anti-CAND1 antibody [EPR14242(B)] - C-terminal
    See all CAND1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14242(B)] to CAND1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human CAND1 aa 1200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q86VP6

  • Positive control

    • Jurkat cells, HeLa cells, Human prostatic hyperplasia tissue, Saos 2 cell line lysate, 293T cell line lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14242(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 136 kDa).
IHC-P 1/50 - 1/100.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
IP 1/40 - 1/60.

Target

  • Function

    Enhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins.
  • Sequence similarities

    Belongs to the CAND family.
    Contains 27 HEAT repeats.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAND 1 antibody
    • Cand1 antibody
    • CAND1_HUMAN antibody
    • Cullin associated and neddylation dissociated 1 antibody
    • Cullin associated and neddylation dissociated protein 1 antibody
    • Cullin associated NEDD8 dissociated protein 1 antibody
    • Cullin-associated and neddylation-dissociated protein 1 antibody
    • Cullin-associated NEDD8-dissociated protein 1 antibody
    • DKFZP434M1414 antibody
    • FLJ10114 antibody
    • FLJ10929 antibody
    • FLJ38691 antibody
    • FLJ90441 antibody
    • KIAA0829 antibody
    • p120 CAND1 antibody
    • TATA binding protein interacting protein 120A antibody
    • TBP interacting protein antibody
    • TBP interacting protein of 120 kDa A antibody
    • TBP Interacting Protein TIP120A antibody
    • TBP-interacting protein 120A antibody
    • TBP-interacting protein of 120 kDa A antibody
    • TIP120 antibody
    • TIP120 protein antibody
    • TIP120A antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling CAND1 with ab181216 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution and counterstained with Dapi (blue).

  • All lanes : Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216) at 1/50000 dilution

    Lane 1 : 293T cell line lysate
    Lane 2 : HeLa cell line lysate
    Lane 3 : Jurkat cell line lysate
    Lane 4 : Saos 2 cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 136 kDa

  • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling CAND1 with ab181216 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Western blot analysis of immunoprecipitation pellet from Hela cell lysate immunoprecipitated using ab181216 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling CAND1 with ab181216 at 1/20 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab181216 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 14 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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