Overview

  • Product name
    Anti-Candida albicans antibody (FITC)
    See all Candida albicans primary antibodies
  • Description
    Rabbit polyclonal to Candida albicans (FITC)
  • Host species
    Rabbit
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Recognizes numerous proteins in a soluble C. albicans extract (IEP). Has not been absorbed and does cross-react with other yeasts. Negative against human serum, urine and spinal fluid.
  • Tested applications
    Suitable for: Double Immunodiffusion, Counter Current Immunoelectrophoresis, ICC/IF, ELISAmore details
  • Species reactivity
    Has not been absorbed and does react with other yeasts.
  • Immunogen

    Candida albicans, type A (ATCC #32354).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Protein A chromatography purified IgG fraction covalently coupled with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Double Immunodiffusion Use at an assay dependent dilution.
Counter Current Immunoelectrophoresis Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Candida albicans is the most frequently isolated fungal pathogen of humans, affecting immunocompromised patients ranging from premature infants to AIDS sufferers. Systemic infections have an attributed mortality of 30-50%. C. albicans is a diploid organism which has eight sets of homologous chromosomes. It has a genome of approximately 16 Mb (haploid), about 30% greater than S. cerevisiae (baker’s yeast).
  • Alternative names
    • C albicans antibody
    • Thrush antibody

References

This product has been referenced in:
  • Drummond RA  et al. CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System. PLoS Pathog 11:e1005293 (2015). Flow Cyt ; Human . Read more (PubMed: 26679537) »
See 1 Publication for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Question
Answer

Per the lab ab20005 does stain C. albicans cells, but usually the FITC version (ab21164) is used. The biotin version should still work well when used with Streptavidin-FITC instead of a secondary FITC-labelled antibody, and using biotin-streptavidin should give you some signal amplification.

The lab recommends a suspension of the culture be dried down at room temperature on the slide. Then a 1:200-1:500 dilution of the antibody applied or thirty minutes at room temperature and washed. The appropriate dilution of the secondary-FITC reagent (as recommended by the manufacturer) is applied for thirty minutes at room temperature. Finally the slide is washed again and mounting medium + coverslip is applied for viewing.

If you need the Streptavidin-FITC conjugate, we sell this under catalog number ab136201.

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Answer

Thank you for contacting us. I am very glad to hear that this product worked so well in FACS.

After submitting the Abreview your code will become 'active'. When you place your next order use the normal system costs and input this code. The code will be checked by our customer service group to determine its activity, e.g. the Abreview has been submitted. If the code is active, the primary antibody will receive a 100% discount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

I am glad that you have decided to take advantage of our 100% Abreview program. To place an Abreview you will need to go to the webpage for the product and click on the 'ABREVIEW' tab just below the shipping information. This tab will contain a link to "Submit an Abreview"

I have provided a link here as well:https://www.abcam.com/index.html?pageconfig=your_reviews&intAbID=53892&strAccessMode=public_create



I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for contacting us. Ab53892 is the only product of these three which is against the extracelluar region specifically. I have created an Abreview discount code for you should you desire to use it. There is not obligation to do so. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. I am very pleased to hear you would like to accept our offer and test ab53892 in FC. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for FC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your patience as I contacted the lab. The staining pattern forab53892 is extracellular. As to our testing program, you are correct. You will receive a code good for one free antibody of your choice which will become active once you have submitted an Abreview for the antibody which you have tested. I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

Therefore, I can offer a discount off a future purchase if you buyab20005 and/or ab21164 and test in FCand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY for antibody tested.


If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testab20005 and/or ab21164 and test in FC. I will then send a discount code. This code must be issued before purchase so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in FC.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if an product turns out to be unsuitable for FC, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting Abcam and for your interest in our Anti-Candida albicans antibodies.


Catalog #s ab2005 and ab21164 are polyclonal antibodies and therefore will recognize multiple epitopes on multiple proteins. The immunogen was a lysate of Candida albicans. Most of the immunogen proteins were cytoplasmic, but the antibody has been shown to stain intact organisms fixed on slides.


If you are interested, I am happy to offer a testing discount in which you would purchase ab20005 and/or ab21164 and test in FC. Then, submit your results in the form of an Abreview. You would then have a discount for a free antibody on a future order (1 discount code per antibody tested and reviewed). Please let me know if you would be interested.


I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions.

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Answer

Thank you for your email. The only non-conjugated Candida albicans antibody that we have is ab23368 (Mouse monoclonal [6401] to Candida albicans). We don't have an unconjugated polyclonal antibody. For ab20005, ab20028 and ab21164, the antibodies will cross-react with yeasts other than Candida. Ab23368 reacts with Candida albicans and hasn't been tested in other species. The procedure used to prepare the soluble extract is considered proprietary. Please contact us again if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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