For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
|Sample type||Average %||Range|
|Serum||> 85||% - %|
Abcam’s Canine Albumin ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Albumin in biological samples of dog.
In this assay the Albumin present in samples reacts with the anti- Albumin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- Albumin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Albumin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Albumin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Albumin in the test sample. The quantity of Albumin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|100X HRP-conjugated anti-dog Albumin antibody||1 x 150µl|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Chromogen Substrate Solution||1 x 12ml|
|Dog Albumin Calibrator (Lyophilized)||1 vial|
|Dog Albumin ELISA Microplate||1 unit|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab157695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab157695 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"