• Product name

    Canine Alpha 1 Acid Glycoprotein / AGP ELISA Kit
    See all Alpha 1 Acid Glycoprotein/AGP kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.706 ng/ml
  • Range

    6.17 ng/ml - 1500 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 6.17ng/ml - 1500ng/ml

  • Assay time

    1h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Dog
  • Product overview

    Alpha 1 Acid Glycoprotein / AGP in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Alpha 1 Acid Glycoprotein / AGP protein in dog biological fluids.

  • Notes

    In this assay the Alpha 1 Acid Glycoprotein / AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein / AGP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein / AGP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein / AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein / AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein / AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein / AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

    Alpha 1 Acid Glycoprotein / AGP is a 52 kDa serine protease inhibitor (serpin) in blood, which protects tissue from enzymes from inflammatory cells, especially elastase. In certain acute phase inflammatory reactions, Alpha 1 Acid Glycoprotein / AGP is elevated in order to limit the damage caused by activated neutrophil granulocytes and their enzyme elastase. Disorders of Alpha 1 Acid Glycoprotein / AGP include Alpha 1 Acid Glycoprotein / AGP deficiency, a hereditary disorder that can lead to severe tissue breakdown during inflammation. This may result in pulmonary emphysema and liver cirrhosis, in severe cases genetic variants of Alpha 1 Acid Glycoprotein / AGP do occur.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)



Our Abpromise guarantee covers the use of ab205082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab205082 Dog Alpha 1 Acid Glycoprotein / AGP ELISA Kit.



ab205082 has not yet been referenced specifically in any publications.

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