Overview

  • Product name

  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 7.88%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 7.18%
  • Sample type

    Serum, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    20 pg/ml
  • Range

    0.08 ng/ml - 5 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102.61 102.54% - 102.67%
    1X Cell Extraction Buffer PTR 101.35 96.12% - 111.33%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Dog
    Does not react with: Mouse, Hamster
  • Product overview

    Abcam’s Canine CKM in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of canine CKM protein in canine serum and tissue extracts.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity:

    Samples diluted in Sample Diluent NS – 20 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR – 50 pg/mL

  • Notes

    Creatine kinase (CK) catalyzes creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and producing adenosine diphosphate (ADP). This process is reversible, thus PCr and ADP can be used to generate ATP and creatine. There are three known cytoplasmic isoenzymes (CKMM- skeletal muscle, CKMB-myocardium, and CKBB- nervous tissue). Phosphocreatine serves as an energy storage for rapid regeneration of ATP. Creatine is used in tissues with high energy demands, such as skeletal muscle, heart, and brain.

    Expression of CKM in serum are based on muscle mass, age, gender, race, and physical activity. Skeletal muscle expresses approximately 98% CKMM and 1% CKMB, myocardium expresses 70% CKMM and 25-30% CKMB, and neural tissue expresses a majority of CKBB.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab197749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab197749 has not yet been referenced specifically in any publications.

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