Key features and details
- Sensitivity: 3 pg/ml
- Range: 600 pg/ml - 2.46 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Product nameCanine Decorin ELISA Kit
See all Decorin kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range600 pg/ml - 2.46 pg/ml
Sample specific recovery Sample type Average % Range Serum 124.1 103% - 143% Plasma 114.6 91% - 140% Cell culture media 107.7 100% - 125%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Dog
Canine Decorin ELISA Kit (ab273186) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Decorin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Canine Decorin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine Decorin Antibody 2 vials Canine Decorin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionMay affect the rate of fibrils formation.
Involvement in diseaseDefects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Bone proteoglycan II
Our Abpromise guarantee covers the use of ab273186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab273186 has not yet been referenced specifically in any publications.