Key features and details
- Sensitivity: 0.13 ng/ml
- Range: 0.102 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Product nameCanine GM-CSF ELISA Kit
See all GM-CSF kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.102 ng/ml - 25 ng/ml
Sample specific recovery Sample type Average % Range Serum 100.5 78% - 127% Plasma 85.19 69% - 108% Cell culture media 87.25 69% - 110%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Dog
Canine GM-CSF ELISA Kit (ab273187) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine GM-CSF in serum (Canine GM-CSF concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Canine GM-CSF coated on a 96- well plate. Standards and samples are pipetted into the wells and Canine GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- Canine GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Canine GM-CSF coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Canine GM-CSF Antibody 2 vials Canine GM-CSF Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Sequence similaritiesBelongs to the GM-CSF family.
- Information by UniProt
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
Our Abpromise guarantee covers the use of ab273187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab273187 has not yet been referenced specifically in any publications.