Overview

  • Product name

    Canine Haptoglobin ELISA Kit
    See all Haptoglobin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 2.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 11%
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    > 0.2 ng/ml
  • Range

    0.313 ng/ml - 20 ng/ml
  • Recovery

    = 97 %

  • Assay time

    4h 0m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Dog
  • Product overview

    Abcam’s Haptoglobin Canine in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit designed for the quantitative measurement of Haptoglobin levels in plasma and cell culture supernatants.

    A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Haptoglobin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Haptoglobin captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Canine Haptoglobin Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Haptoglobin Microplate (12 x 8 well strips) 1 unit
    Haptoglobin Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Defects in HP are the cause of anhaptoglobinemia (AHP) [MIM:614081]. AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • binding peptide
    • BP
    • haptoglobin alpha 1S beta
    • haptoglobin alpha 2FS beta
    • haptoglobin alpha polypeptide
    • Haptoglobin beta chain
    • haptoglobin beta polypeptide
    • HP
    • HP2ALPHA2
    • HPA1S
    • HPT_HUMAN
    • Zonulin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab137978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab137978

Protocols

References

ab137978 has not yet been referenced specifically in any publications.

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