Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.1 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Product nameCanine IFN-gamma ELISA Kit (IFNG)
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.1 ng/ml - 25 ng/ml
Sample specific recovery Sample type Average % Range Serum 77.11 69% - 85% Plasma 81.29 68% - 95% Cell culture media = 73.83 68% - 99%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Dog
Does not react with: Mouse, Rat, Horse, Cow, Cat, Human, Pig
Abcam’s IFN gamma canine ELISA Kit (ab193684) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine IFN gamma in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for canine IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IFN gamma present in the samples. The wells are washed and biotinylated anti-canine IFN gamma antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IFN gamma bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Canine IFN-gamma Detection Antibody 2 vials Canine IFN-gamma Standards (lyophilized) 2 vials Pre-coated Canine IFN-gamma Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
ab193684 has not yet been referenced specifically in any publications.