Key features and details
- Sensitivity: 0.055 ng/ml
- Range: 0.051 ng/ml - 12.5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Product nameCanine IGFBP2 ELISA Kit
See all IGFBP2 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.051 ng/ml - 12.5 ng/ml
Sample specific recovery Sample type Average % Range Serum 105.3 97% - 112% Plasma 98.77 91% - 104% Cell culture media 87.73 74% - 104%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Dog
Canine IGFBP2 ((Insulin-like Growth Factor Binding Protein 2) ELISA Kit (ab273184) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IGFBP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 550X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Canine IGFBP2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine IGFBP2 Antibody 2 vials Canine IGFBP2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Sequence similaritiesContains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
DomainThe C-terminus is required for IGF-binding and growth inhibition.
- Information by UniProt
- BP 2
- IBP 2
Our Abpromise guarantee covers the use of ab273184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab273184 has not yet been referenced specifically in any publications.