Overview

  • Product name

    Canine IgG ELISA Kit
    See all IgG kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma, Other biological fluids
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    5.358 ng/ml
  • Range

    0.025 µg/ml - 245.3 µg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Dog
  • Product overview

    Abcam’s IgG Dog ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in biological samples of dog. 

     In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-dog IgG antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Dog IgG Calibrator (Lyophilized) 1 vial
    Dog IgG ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas

  • Cellular localization

    Secreted
  • Alternative names

    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Applications

Our Abpromise guarantee covers the use of ab157701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157701 IgG Dog ELISA kit

Protocols

References

ab157701 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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