Overview

  • Product name

    Canine IL-10 ELISA Kit
    See all IL-10 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.1 ng/ml
  • Range

    0.1 ng/well - 80 ng/well
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 116.3 94% - 138%
    Plasma 120.1 93% - 149%
    Cell culture media 95.6 77% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Dog
  • Product overview

    Abcam’s IL-10 Canine ELISA Kit (ab193685) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine IL-10 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for canine IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-10 present in the samples. The wells are washed and biotinylated anti-canine IL-10 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-10 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    350X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent D 1 x 15ml
    5X Assay Diluent B 1 x 15ml
    Biotinylated Canine IL-10 Detection Antibody 2 vials
    Canine IL-10 Standards (lyophilized) 2 vials
    Pre-coated canine IL-10 Microplate (12 strips by 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • T-cell growth inhibitory factor
    • TGIF
    see all

Applications

Our Abpromise guarantee covers the use of ab193685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab193685 has not yet been referenced specifically in any publications.

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