• Product name

    Canine IL8 ELISA Kit (Interleukin-8)
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 6 pg/ml
  • Range

    6.1 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 110.2 102% - 118%
    Plasma 103.2 84% - 122%
    Cell culture media 128.2 122% - 132%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Dog
  • Product overview

    Abcam’s Interleukin-8 (IL-8) Canine ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of dog IL-8 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for dog IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-dog IL-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Detection Antibody IL-8 (biotinylated anti-Canine IL-8) 2 vials
    IL-8 Microplate (12 strips x 8 wells) coated with anti canine IL-8 1 unit
    IL-8 Standard canine (recombinant) (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Subunit structure: Homodimer. Induction: By ER stress in a DDIT3/CHOP-dependent manner. Post-translational modification: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation. Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

  • Alternative names

    • alveolar macrophage chemotactic factor I
    • beta endothelial cell derived neutrophil activating peptide
    • beta thromboglobulin like protein
    • C X C motif chemokine ligand 8
    • chemokine (C X C motif) ligand 8
    • chemokine (CXC motif) ligand 8
    • CXCL 8
    • CXCL8
    • emoctakin
    • GCP 1
    • GCP1
    • granulocyte chemotactic protein 1
    • IL 8
    • IL8
    • interleukin 8
    • LECT
    • LUCT
    • lung giant cell carcinoma derived chemotactic protein
    • lymphocyte derived neutrophil activating peptide
    • LYNAP
    • MDNCF
    • MONAP
    • monocyte derived neutrophil activating peptide
    • monocyte derived neutrophil chemotactic factor
    • NAF
    • NAP 1
    • NAP1
    • neutrophil activating peptide 1
    • small inducible cytokine subfamily B, member 8
    • T cell chemotactic factor
    • tumor necrosis factor induced gene 1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab155465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab155465



This product has been referenced in:

  • Avazi DO  et al. Evaluation of levels of interleukin-6, interleukin-8 and some haematologic parameters of dogs with cutaneous wounds. Cytokine 113:128-138 (2019). Read more (PubMed: 30539777) »
See 1 Publication for this product

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