Overview

  • Product name

    Canine KIM-1 ELISA Kit
    See all TIM 1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.034 ng/ml
  • Range

    0.031 ng/ml - 1 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 0.031ng/ml - 1ng/ml

  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Dog
  • Product overview

    Kidney Injury Molecule-1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kidney Injury Molecule-1 protein in dog biological fluids.

  • Notes

    In this assay the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Detection Antibody Conjugate 1 x 150µl
    100X HRP Streptavidin 1 x 150µl
    1X Diluent Concentrate 1 x 60ml
    20X Wash Buffer Concentrate 1 x 50ml
    Anti-Dog KIM-1 ELISA Microplate 1 unit
    Chromogen Substrate Solution 1 x 12ml
    Dog KIM-1 Calibrator (Lyophilized) 1 vial
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificity

    Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • CD365
    • HAVCR
    • HAVCR 1
    • HAVcr-1
    • Havcr1
    • Hepatitis A virus cellular receptor 1
    • Kidney injury molecule 1
    • KIM 1
    • KIM-1
    • T cell immunoglobin domain and mucin domain protein 1
    • T cell immunoglobulin mucin family member 1
    • T cell immunoglobulin mucin receptor 1
    • T-cell immunoglobulin and mucin domain-containing protein 1
    • T-cell membrane protein 1
    • TIM
    • TIM-1
    • TIM1
    • TIMD 1
    • TIMD-1
    • TIMD1
    • TIMD1_HUMAN
    see all

Applications

Our Abpromise guarantee covers the use of ab205084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab205084 Dog KIM-1 ELISA Kit.

Protocols

References

ab205084 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Test canine KIM-1 ELISA kit with feline urine

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Feline urine sample was tested with canine KIM-1ELISA kit for cross reactivity test. ELISA was carried out with some modifications (dilution factor and incubation time) and we could quantify the level of feline KIM-1. Overall our sample concentration was low .
All kit procedure was really easy to follow and color pigment in the diluent solution helps to distinguish each wells when I add sample.

Abcam user community

Verified customer

Submitted Aug 02 2017

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