Overview

  • Product name

    Canine NGAL ELISA Kit
    See all Lipocalin-2 / NGAL kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.01 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 15.6pg/ml - 1000pg/ml

  • Assay time

    3h 20m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Dog
  • Product overview

    NGAL (ab205085) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of NGAL protein in biological samples of dogs.


    In this assay the NGAL present in samples reacts with the anti-NGAL antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-NGAL, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of NGAL in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of NGAL in the test sample. The quantity of NGAL in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Detection Antibody Conjugate 1 x 150µl
    100X HRP Streptavidin 1 x 150µl
    1X Diluent Concentrate 1 x 60ml
    20X Wash Buffer Concentrate 1 x 50ml
    Anti-Dog NGAL ELISA Microplate 1 unit
    Chromogen Substrate Solution 1 x 12ml
    DOG NGAL Calibrator (Lyophilized) 1 vial
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificity

    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • Alternative names

    • 24p3
    • 25 kDa alpha-2-microglobulin related subunit of MMP-9
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
    • HNL
    • Lcn 2
    • Lcn2
    • Lipocalin 2
    • Lipocalin-2
    • Migration stimulating factor inhibitor
    • MSF1
    • MSFI
    • Neutrophil gelatinase associated lipocalin
    • Neutrophil gelatinase-associated lipocalin
    • NGAL
    • NGAL_HUMAN
    • Oncogene 24p3
    • p25
    • Siderocalin
    • Siderocalin LCN2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab205085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab205085 Dog NGAL ELISA Kit.

Protocols

References

ab205085 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Feline urine was tested with canine NGAL ELISA kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Feline urine was tested using this kit. First, 50 fold diluted urine sample was tested following instruction. Most of sample absorbance was below base line. So at the second attempt 5 or 2 fold diluted feline urine was reacted for overnight at 4C. I could obtain values and attached image is the standard curve from overnight reaction.

Abcam user community

Verified customer

Submitted Dec 20 2017

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