Anti-Cannabinoid Receptor I antibody (ab118277)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cannabinoid Receptor I antibody
See all Cannabinoid Receptor I primary antibodies -
Description
Goat polyclonal to Cannabinoid Receptor I -
Host species
Goat -
Specificity
ab118277 is expected to recognize reported isoform a (NP_057167.2) only. Reported variants represent identical protein: NP_001153731.1, NP_001153732.1, NP_057167.2, NP_001153730.1, NP_001153698.1 -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Cat, Dog
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Immunogen
Synthetic peptide:
SNDIQYEDIKGDMAS
with a Cysteine residue linker, corresponding to N terminal amino acids 25-39 of Human Cannabinoid Receptor I (NP_057167.2). -
Positive control
- A549 and NIH 3T3 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab118277 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab118277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100299449 Cow
- Entrez Gene: 481913 Dog
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: O02777 Cat
- SwissProt: P21554 Human
see all -
Alternative names
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
Images
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Western blot - Anti-Cannabinoid Receptor I antibody (ab118277)Anti-Cannabinoid Receptor I antibody (ab118277) at 1 µg/ml + NIH 3T3 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 1 hour
Protocols
Datasheets and documents
References
ab118277 has not yet been referenced specifically in any publications.
