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  1. Link

    cannabinoid-receptor-i-antibody-ab118277.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Cannabinoid Receptor
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Anti-Cannabinoid Receptor I antibody (ab118277)

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Western blot - Anti-Cannabinoid Receptor I antibody (ab118277)

    Key features and details

    • Goat polyclonal to Cannabinoid Receptor I
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Cannabinoid Receptor I antibody
      See all Cannabinoid Receptor I primary antibodies
    • Description

      Goat polyclonal to Cannabinoid Receptor I
    • Host species

      Goat
    • Specificity

      ab118277 is expected to recognize reported isoform a (NP_057167.2) only. Reported variants represent identical protein: NP_001153731.1, NP_001153732.1, NP_057167.2, NP_001153730.1, NP_001153698.1
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rat, Cow, Cat, Dog
    • Immunogen

      Synthetic peptide within Human Cannabinoid Receptor I aa 1-100 (N terminal) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Accession: NP_057167.2

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: A549 and NIH/3T3 cell lysates.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 99% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab118277 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • GPCR
      • Cannabinoid Receptor
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipoprotein metabolism
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human Cannabinoid Receptor I protein (ab158150)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab118277 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

    1 hour primary incubation is recommended for this product.

    Notes
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P21554 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 100299449 Cow
      • Entrez Gene: 481913 Dog
      • Entrez Gene: 1268 Human
      • Entrez Gene: 12801 Mouse
      • Entrez Gene: 25248 Rat
      • Omim: 114610 Human
      • SwissProt: O02777 Cat
      • SwissProt: P21554 Human
      • SwissProt: P47746 Mouse
      • SwissProt: P20272 Rat
      • Unigene: 709067 Human
      • Unigene: 75110 Human
      • Unigene: 7992 Mouse
      • Unigene: 222240 Rat
      • Unigene: 89774 Rat
      see all
    • Alternative names

      • CANN6 antibody
      • Cannabinoid receptor 1 antibody
      • CB-R antibody
      • CB1 antibody
      • CB1A antibody
      • CB1K5 antibody
      • CB1R antibody
      • Central cannabinoid receptor antibody
      • CNR antibody
      • CNR1 antibody
      • CNR1_HUMAN antibody
      • OTTHUMP00000016838 antibody
      • OTTHUMP00000214579 antibody
      see all

    Images

    • Western blot - Anti-Cannabinoid Receptor I antibody (ab118277)
      Western blot - Anti-Cannabinoid Receptor I antibody (ab118277)
      All lanes : Anti-Cannabinoid Receptor I antibody (ab118277) at 0.3 µg/ml

      Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate in RIPA buffer
      Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate in RIPA buffer

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 53 kDa



      Primary incubation for 1 hour. Detected by chemiluminescence.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab118277? Please let us know so that we can cite the reference in this datasheet.

    ab118277 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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