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  1. Link

    cannabinoid-receptor-i-antibody-ab23703.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Cannabinoid Receptor
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Anti-Cannabinoid Receptor I antibody (ab23703)

  • Datasheet
  • SDS
Reviews (20)Q&A (14)References (79)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)

Key features and details

  • Rabbit polyclonal to Cannabinoid Receptor I
  • Suitable for: IHC-P, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG

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AMG 9810, TRPV1 receptor antagonist (ab145874)
Protein
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Recombinant Human Cannabinoid Receptor I protein (ab158150)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cannabinoid Receptor I antibody
    See all Cannabinoid Receptor I primary antibodies
  • Description

    Rabbit polyclonal to Cannabinoid Receptor I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Cow, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide:

    MSVSTDTSAE AL

    , corresponding to C terminal amino acids 461-472 of Human Cannabinoid Receptor I(Peptide available as ab50542.)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Cannabinoid Receptor
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Cannabinoid Receptor I peptide (ab50542)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cannabinoid Receptor I protein (ab158150)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab23703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (7)
Use at an assay dependent concentration.
ICC/IF (4)
Use at an assay dependent concentration.
IHC-Fr (2)
Use at an assay dependent concentration.

Detection with an ABC system is recommended.

Notes
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Detection with an ABC system is recommended.

Target

  • Function

    Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P21554 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 100299449 Cow
    • Entrez Gene: 481913 Dog
    • Entrez Gene: 1268 Human
    • Entrez Gene: 12801 Mouse
    • Entrez Gene: 25248 Rat
    • Omim: 114610 Human
    • SwissProt: Q5IS73 Chimpanzee
    • SwissProt: P21554 Human
    • SwissProt: P47746 Mouse
    • SwissProt: P20272 Rat
    • Unigene: 709067 Human
    • Unigene: 75110 Human
    • Unigene: 7992 Mouse
    • Unigene: 222240 Rat
    • Unigene: 89774 Rat
    see all
  • Alternative names

    • CANN6 antibody
    • Cannabinoid receptor 1 antibody
    • CB-R antibody
    • CB1 antibody
    • CB1A antibody
    • CB1K5 antibody
    • CB1R antibody
    • Central cannabinoid receptor antibody
    • CNR antibody
    • CNR1 antibody
    • CNR1_HUMAN antibody
    • OTTHUMP00000016838 antibody
    • OTTHUMP00000214579 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)
    Immunoperoxidase staining of CA region of rat hippocampus with the Cannabinoid Receptor I antibody (ab23703, 4ug/ml). the majority of the protein is localized in the pre-synaptic axons, shown as brown particulaes. A few neurons also show cytoplasm staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)Courtesy of an annonymous Abreview

    ab23703 staining Cannabinoid Receptor I in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (79)

Publishing research using ab23703? Please let us know so that we can cite the reference in this datasheet.

ab23703 has been referenced in 79 publications.

  • He XL  et al. Solid lipid nanoparticles loading with curcumin and dexanabinol to treat major depressive disorder. Neural Regen Res 16:537-542 (2021). PubMed: 32985484
  • Lah TT  et al. Cannabigerol Is a Potential Therapeutic Agent in a Novel Combined Therapy for Glioblastoma. Cells 10:N/A (2021). PubMed: 33562819
  • Daddam JR  et al. Phosphoproteomic Analysis of Subcutaneous and Omental Adipose Tissue Reveals Increased Lipid Turnover in Dairy Cows Supplemented with Conjugated Linoleic Acid. Int J Mol Sci 22:N/A (2021). PubMed: 33810070
  • Rodriguez JL  et al. Involvement of the endocannabinoid system in the inhibition of Sindbis virus replication: a preliminary study. J Cannabis Res 3:10 (2021). PubMed: 33892823
  • Lee DI  et al. Cannabinoid Receptor Agonist Inhibits Atrial Electrical Remodeling in a Tachypaced Ex Vivo Rat Model. Front Pharmacol 12:642398 (2021). PubMed: 33967775
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

Filter by Application

Filter by Species

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1-10 of 20 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (pituitary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrus pH 6.0
Permeabilization
No
Specification
pituitary gland
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 28 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Brain)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Hyaluronidase
Permeabilization
Yes - BSA
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20%
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 18 2019

Immunohistochemistry (Frozen sections) abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Horse Tissue sections (dorsal root ganglion)
Permeabilization
Yes - Triton
Specification
dorsal root ganglion
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 23 2019

Immunohistochemistry (Frozen sections) abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Cat Tissue sections (gastrointestinal tract)
Permeabilization
Yes - Triton
Specification
gastrointestinal tract
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Roberto Chiocchetti

Verified customer

Submitted Dec 04 2018

Western blot abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Chicken Cell lysate - whole cell (Cerebellar granule neurons from embryonic day 17 c)
Gel Running Conditions
Non-reduced Denaturing (4-10 %)
Loading amount
25 µg
Treatment
Various chemicals, including Paracetamol, AM-404, Rimonabant
Specification
Cerebellar granule neurons from embryonic day 17 c
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Mussie Ghezu Hadera

Verified customer

Submitted Sep 18 2018

Flow Cytometry abreview for Anti-Cannabinoid Receptor I antibody

Average
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (Spleen cells)
Permeabilization
No
Gating Strategy
live cells
Specification
Spleen cells
Preparation
Cell harvesting/tissue preparation method: cell culture (RPMI)
Sample buffer: PBS 1x
Fixation
Fresh cells
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Coral González García

Verified customer

Submitted Aug 21 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cannabinoid Receptor I antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Sample
Cat Tissue sections (Felis catus brain parietal cortex)
Specification
Felis catus brain parietal cortex
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 05 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cannabinoid Receptor I antibody

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
10% buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 22 2014

Western blot abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Sample
Rat Tissue lysate - whole (bladder)
Specification
bladder
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Eun-Young Park

Verified customer

Submitted Jul 18 2013

Western blot abreview for Anti-Cannabinoid Receptor I antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (mouse brain - post nuclear supernatant)
Loading amount
20 µg
Specification
mouse brain - post nuclear supernatant
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 05 2013

1-10 of 20 Abreviews

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