Anti-Cannabinoid Receptor I antibody (ab23703)
Rabbit polyclonal Cannabinoid Receptor I antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Dog, Human. Cited in 36 publication(s). Independently reviewed in 17 review(s).
- Datasheet
- References (41)
- Protocols
Overview
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Product nameAnti-Cannabinoid Receptor I antibody
See all Cannabinoid Receptor I primary antibodies -
DescriptionRabbit polyclonal to Cannabinoid Receptor I
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Dog, Human
Predicted to work with: Cow, Chimpanzee, Macaque monkey
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Immunogen
Synthetic peptide:
MSVSTDTSAE AL
, corresponding to C terminal amino acids 461-472 of Human Cannabinoid Receptor I(Peptide available as ab50542.) -
Positive control
- Human cerebellum or murine brain sections.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab23703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).Can be blocked with Cannabinoid Receptor I peptide (ab50542). | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | 1/300. Detection with an ABC system is recommended. | |
| ICC/IF | 1/50. |
Target
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FunctionInvolved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
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Tissue specificityWidely expressed.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 100299449 Cow
- Entrez Gene: 481913 Dog
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: Q5IS73 Chimpanzee
- SwissProt: P21554 Human
see all -
Alternative names
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
Images
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Western blot - Anti-Cannabinoid Receptor I antibody (ab23703)Anti-Cannabinoid Receptor I antibody (ab23703) + Human cerebellum at 30 µg
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Secondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)Immunoperoxidase staining of CA region of rat hippocampus with the Cannabinoid Receptor I antibody (ab23703, 4ug/ml). the majority of the protein is localized in the pre-synaptic axons, shown as brown particulaes. A few neurons also show cytoplasm staining. -
Immunohistochemistry (Frozen sections) - Anti-Cannabinoid Receptor I antibody (ab23703)Image from Laurén HB et al, PLoS One. 2010 May 20;5(5):e10733, Fig 5.ab23703 staining Cannabinoid Receptor I in rat brain tissue by Immunohistochemistry (Frozen sections). After fixation in 4% PFA in PBS (pH 7.4), brains were cryoprotected in 30% sucrose in PBS at +4°C, frozen, and thereafter kept at -80°C until used. For the immunostaining, brains were cryosectioned in 30 µm slices, collected in PBS (pH 7.4), and processed in a free-floating system. Slices were first incubated in the blocking solution (BS) containing 2% bovine serum albumin, 2% goat serum, and 0.1% Triton X-100 in PBS (pH 7.4) for 1 hour at room temperature, and thereafter with the primary antibody for 24–48 hours at +4°C in BS at a 1/1000 dilution. After washing in PBS, slices were incubated with the biotin-conjugated secondary antibody (1/4000) in BS, rinsed with PBS, and incubated with the avidin-peroxidase conjugate in BS for 1 hour at room temperature. The staining was detected using 3,3-diaminobenzidinetetrahydrochloride as a chromogen. -
Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody (ab23703)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of mouse cultured hippocampal neurons, staining Cannabinoid Receptor I with ab23703.
Cells were fixed with paraformaldehyde, permeabilized with 0.05% Saponin and blocked with 10% fetal calf serum for 2 hous at 4°C. Samples were incubated with primary antibody (1/200 in 0.05% Saponin + fetal calf serum) for 12 hours at 23°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)Courtesy of an annonymous Abreviewab23703 staining Cannabinoid Receptor I in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Ballesta A et al. Bupropion, a possible antidepressant without negative effects on alcohol relapse. Eur Neuropsychopharmacol 29:756-765 (2019). Read more (PubMed: 31064683) »
- Kasacka I et al. Comparative evaluation of cannabinoid receptors, apelin and S100A6 protein in the heart of women of different age groups. BMC Cardiovasc Disord 18:190 (2018). Read more (PubMed: 30286717) »
