Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic stelate cells)
Specification
pancreatic stelate cells
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH=6
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/50
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Vector Lab Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000

Additional data

Additional Notes
Cannabinoid Receptor I is expressed by pancreatic stellate cells as expected.

Cannabinoids Reduce Markers of Inflammation and Fibrosis in Pancreatic Stellate Cells by Christoph W. Michalski et al, 2008, PlosOne

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Submitted Jan 28 2011

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