Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cannabinoid Receptor I antibody

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain sections)
Specification
mouse brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Triton during block and staining
Blocking step
PBS, 10%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS, 1%Horse serum, 0,2%BSA, 0,3%Triton

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit AlexaFluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400

Additional data

Additional Notes
Images are from dentate gyrus and CA1 area of the hippocampal formation.
Nuclei were stained with DAPI.

Abcam user community

Verified customer

Submitted Jan 07 2013

Sign up