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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (mouse brain sections)
mouse brain sections
Antigen retrieval step
Yes - Triton during block and staining
PBS, 10%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS, 1%Horse serum, 0,2%BSA, 0,3%Triton
Non-Abcam antibody was used: goat anti rabbit AlexaFluor488
Host species: Goat
Conjugation: Alexa Fluor® 488
Images are from dentate gyrus and CA1 area of the hippocampal formation.
Nuclei were stained with DAPI.
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Submitted Jan 07 2013