Immunocytochemistry/ Immunofluorescence abreview for Anti-Cannabinoid Receptor I antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cultured hippocampal neurons)
Specification
cultured hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,05% (w/v) saponin in PBS
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Other product details

Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 0,05% (w/v) Saponin/PBS + 10% FCS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit AlexaFluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400

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Submitted Jan 07 2013

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