Western blot abreview for Anti-Cannabinoid Receptor I antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (mouse brain - post nuclear supernatant)
Loading amount
20 µg
Specification
mouse brain - post nuclear supernatant
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 0,1% Tween-20

Secondary antibody

Name
Non-Abcam antibody was used: Peroxidase goat anti rabbit IgG-antibody PI-1000 V
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: ~60 kDa Non-specific: several kDa

Additional data

Additional Notes
Excising the area with the expected bands from the Blots increases detection efficiency.

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Verified customer

Submitted Feb 05 2013

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