Western blot abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2, 293T)
Loading amount
20 µg
Specification
HepG2, 293T
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C

Other product details

Dilution
1/200
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: HRP-conjugated anti rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL+
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 65 kDa Non-specific: 37 kDa
Positive control
-
Negative control
-

Mr. Dongil Kim

Verified customer

Submitted Feb 01 2013

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