Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cannabinoid Receptor I antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Sample
Cat Tissue sections (Felis catus brain parietal cortex)
Specification
Felis catus brain parietal cortex
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/80

Secondary antibody

Name
Non-Abcam antibody was used: Vector Panspecific biotynilated antibody BA-1300
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/100

Abcam user community

Verified customer

Submitted Nov 05 2014

Sign up