Western blot abreview for Anti-Cannabinoid Receptor I antibody

Excellent
Abreviews
Application
Western blot
Sample
Chicken Cell lysate - whole cell (Cerebellar granule neurons from embryonic day 17 c)
Gel Running Conditions
Non-reduced Denaturing (4-10 %)
Loading amount
25 µg
Treatment
Various chemicals, including Paracetamol, AM-404, Rimonabant
Specification
Cerebellar granule neurons from embryonic day 17 c
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS buffer with 1 % Tween 5% dry milk
Dilution
1/200

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Luminata classico
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50 kDa

Dr. Mussie Ghezu Hadera

Verified customer

Submitted Sep 18 2018

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