Overview

  • Product name

    Anti-Cannabinoid Receptor I antibody
    See all Cannabinoid Receptor I primary antibodies
  • Description

    Rabbit polyclonal to Cannabinoid Receptor I
  • Host species

    Rabbit
  • Specificity

    Detects CB1 from Human and Rat tissues as well as transfected Rat CB1.
  • Tested applications

    Suitable for: ICC, WB, IP, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Human, Ferret
    Predicted to work with: Cat, Chimpanzee, Rhesus monkey
  • Immunogen

    Fusion protein corresponding to Human Cannabinoid Receptor I aa 1-99 (N terminal).

Properties

Applications

Our Abpromise guarantee covers the use of ab3558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/1000. Immunocytochemical staining of CB1 in AtT20 cells transfected with the rat CB1 gene yields a pattern consistent with plasma membrane staining.
WB 1/250. By Western blot, this antibody detects an ~60 kDa protein representing CB1 from rat brain homogenate.
IP Use at an assay dependent concentration. PubMed: 22387618
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20206138

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Quantification of CB1 and CB2 receptor surface expression in primary Human and HaCaT keratinocytes. (A) FL-1 shows autofluorescence (black population), non-specific binding of secondary antibody (dark grey population) and specific immunofluorescence obtained with CB1 (ab3558) and CB2 (ab3561) receptor antibodies, respectively (light grey population). Histogram shows representative measurement (5000 cells counted). (B) Differences in CB receptor expression between primary and HaCaT keratinocytes, showing marked increased expression of CB2 in primary keratinocytes (geo mean corrected for non-specific binding of secondary Ab). Data show mean values ±SEM obtained with two human primary keratinocyte samples and HaCaT cells, each measured three times.

  • ab3558 at a 1:1000 dilution staining Rat CB1 in transfected AtT20 cells by immunocytochemistry.

References

This product has been referenced in:

  • Kleyer J  et al. Cannabinoid receptor trafficking in peripheral cells is dynamically regulated by a binary biochemical switch. Biochem Pharmacol 83:1393-412 (2012). Flow Cyt, IP ; Human . Read more (PubMed: 22387618) »
  • Shen L  et al. The role of peripheral cannabinoid receptors type 1 in rats with visceral hypersensitivity induced by chronic restraint stress. J Neurogastroenterol Motil 16:281-90 (2010). WB ; Rat . Read more (PubMed: 20680167) »
See all 3 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult brain)
Specification
adult brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Mr. Carl Hobbs

Verified customer

Submitted Oct 16 2007

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