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I have answered these questions last time. Every thing is the same. Did you test this antibody before?
Asked on Mar 31 2005
Thank you for your email. Yes, this has been tested and characterized in Western blotting as well as Immunocytochemistry. In Western blotting, this antibody detects an ~60 kDa protein representing CB1 from rat brain homogenate. The bands that you are seeing at 45 and 80 kDa may be non specific and this point I would suggest decreasing the concentration of the primary as well as the incubation period. Also, make sure to run a secondary control to ensure that these bands are not due to your secondary antibody.
Answered on Apr 01 2005