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I used the same membrane to stain the other protein (Pten). I found this membrane works very well. This means my protocol for western blot has no problem. So, I wonder if you have this kind of antibody with different Lot No.
Asked on Apr 05 2005
Thank you for your email and patience. I do suggest decreasing the concentration of the primary as well as the incubation period as the bands you are seeing may be non-specific. If you are using the same secondary with the Pten primary and not getting any additional bands then your secondary is most likely working fine. However, you probably still need to optimize the conditions with ab3558.
Answered on Apr 11 2005