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Thank you for your assistance. I contacted the customer and suggested her to follow your instructions. I asked her about the storage conditions and indeed, she did not notice the indication on the datasheet. However, the antibody did not entirely loose activity. Is it possible that the two very close bands she is seeing are due to transcript variants or post translational modifications? I found 2 variants in the human protein, but no clear indication in the rat case. Thank you,
Asked on Jan 03 2007
Thank you for your message which has been forwarded to me on behalf of Tanya while she is away. It is with regret that we are not able to guarantee this product if it has not been stored as indicated on the datasheet. However, the results indicate that the antibody is still active and it is very likely the bands observed are from splice variants of the cannabinoid receptor 1 protein. There are splice variants of the protein in rat as well as human as indicated in the following reference: Journal of Pharmacology and Experimental Therapeutics. Vol. 282, Issue 3, 1632-1642, 1997 Regional Differences in Cannabinoid Receptor/G-protein Coupling in Rat Brain1. Christopher S. Breivogel et al. The extra band could also result from differences in post-translational modification. I hope this information is helpful. Should the customer have any further questions, please do not hesitate to contact us again.
Answered on Jan 03 2007