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We are using ab3558 and ab3560 for flow cytometry and would like to know which isotype control you recommend.
Asked on Feb 21 2012
Thank you for your inquiry.
The best way to find isotype controls for an antibody is to start at our homepage www.abcam.com. Go to the yellow bar at the top of the page and move the mouse to “Products,” then click on “Isotype Controls”. The direct link is:
Then select the correct isotype control based on the host species as well as the isotype, sub-isotype, conjugation, and application of the primary antibody which you are using.
For example, if the primary antibody is a FITC-conjugated mouse IgG1 for use in flow cytometry, then you will need to choose a FITC-conjugated mouse IgG1 isotype control tested in flow cytometry.
Please note that most isotype controls are monoclonal. They are not suitable for use with polyclonal antibodies as these contain more than one IgG subclass. However, we do provide several polyclonal isotype controls for use in these circumstances.
I taken the information that you have provided me to determine that the best isotype control in this instance is ab37415 Rabbit IgG- Chip Grade. Should you decide to conjugate a fluorescent tag onto your antibodies however,it is advised that you use a different isotype control which is also conjugated to the same fluorescent label.
Please let me know if have any questions. I would be happy to assist you.
Answered on Feb 21 2012