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Host species: Goat
I have also immunostained this Ab , in parallel with Elphick's, on CB1KO and wt pwax mouse brains: the KO was negative using Elphick's Ab but was positive in all of the same areas as the wt, using this Ab reagent.
I am far from an expert in this field but, after consultation with Dr Scott Graham in NZ and also Professor Elphick my understanding is that it is most likely that this Ab is not detecting CB1 receptor protein.
I have posted pics of Elphick's anti-CB1 Ab on this site: http://www.immunoportal.com/index.php
NB: I have used four types of AR solutions ( and no AR and also varying protease digestions) and they have all failed, relative to Citric acid pH 6 HIER.
Mr. Carl Hobbs
Submitted Oct 16 2007