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  1. Link

    cannabinoid-receptor-i-antibody-ab40860.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Cannabinoid Receptor
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Anti-Cannabinoid Receptor I antibody (ab40860)

  • Datasheet
Reviews (2) Submit a question References (1)

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Western blot - Anti-Cannabinoid Receptor I antibody (ab40860)

    Key features and details

    • Goat polyclonal to Cannabinoid Receptor I
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Recombinant Human Cannabinoid Receptor I protein (ab158150)
    Primary
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    Anti-Cannabinoid Receptor II antibody (ab3561)
    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    View more associated products

    Overview

    • Product name

      Anti-Cannabinoid Receptor I antibody
      See all Cannabinoid Receptor I primary antibodies
    • Description

      Goat polyclonal to Cannabinoid Receptor I
    • Host species

      Goat
    • Specificity

      This antibody is expected to recognise both reported isoforms (NP_057167.2 and NP_149421.1).
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Dog
    • Immunogen

      Synthetic peptide corresponding to Human Cannabinoid Receptor I aa 320-334 (internal sequence). (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1)
      Sequence:

      HTSEDGKVQVTRPDQ

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: HeLa cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • GPCR
      • Cannabinoid Receptor
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipoprotein metabolism
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human Cannabinoid Receptor I protein (ab158150)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab40860 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 54, 63 kDa (predicted molecular weight: 53 kDa).

    A 1 hour primary incubation is recommended for this product. The observed molecular weights correspond to the glycosylated (63 kDa) and unglycosylated (54 kDa) forms as described in the literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343).

    Notes
    WB
    Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 54, 63 kDa (predicted molecular weight: 53 kDa).

    A 1 hour primary incubation is recommended for this product. The observed molecular weights correspond to the glycosylated (63 kDa) and unglycosylated (54 kDa) forms as described in the literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343).

    Target

    • Function

      Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P21554 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 481913 Dog
      • Entrez Gene: 1268 Human
      • Entrez Gene: 12801 Mouse
      • Entrez Gene: 25248 Rat
      • Omim: 114610 Human
      • SwissProt: P21554 Human
      • SwissProt: P47746 Mouse
      • SwissProt: P20272 Rat
      • Unigene: 709067 Human
      • Unigene: 75110 Human
      • Unigene: 7992 Mouse
      • Unigene: 222240 Rat
      • Unigene: 89774 Rat
      see all
    • Alternative names

      • CANN6 antibody
      • Cannabinoid receptor 1 antibody
      • CB-R antibody
      • CB1 antibody
      • CB1A antibody
      • CB1K5 antibody
      • CB1R antibody
      • Central cannabinoid receptor antibody
      • CNR antibody
      • CNR1 antibody
      • CNR1_HUMAN antibody
      • OTTHUMP00000016838 antibody
      • OTTHUMP00000214579 antibody
      see all

    Images

    • Western blot - Anti-Cannabinoid Receptor I antibody (ab40860)
      Western blot - Anti-Cannabinoid Receptor I antibody (ab40860)
      All lanes : Anti-Cannabinoid Receptor I antibody (ab40860) at 1 µg/ml

      Lane 1 : HeLa lysate in RIPA buffer (non-glycosylated)
      Lane 2 : HeLa lysate in RIPA buffer (non-glycosylated) with peptide at 0.5 µg/ml
      Lane 3 : HeLa lysate in RIPA buffer (glycosylated)
      Lane 4 : HeLa lysate in RIPA buffer (glycosylated) with peptide at 0.5 µg/ml

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 53 kDa



      Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab40860? Please let us know so that we can cite the reference in this datasheet.

    ab40860 has been referenced in 1 publication.

    • Kim J  et al. Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid system gene expression in proliferating and differentiating C2C12 myoblasts. Front Physiol 5:100 (2014). Flow Cyt ; Mouse . PubMed: 24711795

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-2 of 2 Abreviews

    Flow Cytometry abreview for Anti-Cannabinoid Receptor I antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (synovial fibroblasts from osteoarthritic person)
    Specification
    synovial fibroblasts from osteoarthritic person
    Preparation
    Cell harvesting/tissue preparation method: cells were detached by accutase (PAA), spun and fixed in 4 % formalin for 20 min. Then, cells were spun and resuspended in 100 µl BD Perm/Wash solution containing 0,5 µg CB1 (ab40860). This was then incubated for 30 min at 4 degrees. Samples were washed with 1ml of BD Perm/Wash buffer and spun again. Then 100 µl of BD Perm/wash were added including 1 µl secondary antibody (donkey anti goat, Santa Cruz Biotech). This was again incubated at 4 degrees for 30 min. After another wash step, cells were resuspended in 4 % Formalin in PBS and analyzed.
    Sample buffer: BD Perm/Wash, 4 % Formaldehyde in PBS
    Fixation
    Formaldehyde
    Permeabilization
    Yes - BD Perm/Wash
    Gating Strategy
    fibroblast culture only
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Torsten Lowin

    Verified customer

    Submitted Apr 16 2009

    Western blot abreview for Anti-Cannabinoid Receptor I antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese Hamster Recombinant protein (CHO cells expressing human CB1 receptor)
    Loading amount
    60000 cells
    Specification
    CHO cells expressing human CB1 receptor
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 26 2008

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