Anti-Cannabinoid Receptor I antibody (ab40860)
Key features and details
- Goat polyclonal to Cannabinoid Receptor I
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cannabinoid Receptor I antibody
See all Cannabinoid Receptor I primary antibodies -
Description
Goat polyclonal to Cannabinoid Receptor I -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms (NP_057167.2 and NP_149421.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog
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Immunogen
Synthetic peptide corresponding to Human Cannabinoid Receptor I aa 320-334 (internal sequence). (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1)
Sequence:HTSEDGKVQVTRPDQ
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Positive control
- WB: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 54, 63 kDa (predicted molecular weight: 53 kDa).
A 1 hour primary incubation is recommended for this product. The observed molecular weights correspond to the glycosylated (63 kDa) and unglycosylated (54 kDa) forms as described in the literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). |
| Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 54, 63 kDa (predicted molecular weight: 53 kDa). A 1 hour primary incubation is recommended for this product. The observed molecular weights correspond to the glycosylated (63 kDa) and unglycosylated (54 kDa) forms as described in the literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). |
Target
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Function
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 481913 Dog
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: P21554 Human
- SwissProt: P47746 Mouse
- SwissProt: P20272 Rat
see all -
Alternative names
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
Images
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Western blot - Anti-Cannabinoid Receptor I antibody (ab40860)All lanes : Anti-Cannabinoid Receptor I antibody (ab40860) at 1 µg/ml
Lane 1 : HeLa lysate in RIPA buffer (non-glycosylated)
Lane 2 : HeLa lysate in RIPA buffer (non-glycosylated) with peptide at 0.5 µg/ml
Lane 3 : HeLa lysate in RIPA buffer (glycosylated)
Lane 4 : HeLa lysate in RIPA buffer (glycosylated) with peptide at 0.5 µg/ml
Lysates/proteins at 35 µg per lane.
Predicted band size: 53 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (1)
ab40860 has been referenced in 1 publication.
- Kim J et al. Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid system gene expression in proliferating and differentiating C2C12 myoblasts. Front Physiol 5:100 (2014). Flow Cyt ; Mouse . PubMed: 24711795
