Anti-Cannabinoid Receptor I antibody (ab40860)

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Overview

  • Product name

    Anti-Cannabinoid Receptor I antibody
    See all Cannabinoid Receptor I primary antibodies

  • Description

    Goat polyclonal to Cannabinoid Receptor I

  • Host species

    Goat

  • Specificity

    This antibody is expected to recognise both reported isoforms (NP_057167.2 and NP_149421.1).

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog

  • Immunogen

    Synthetic peptide corresponding to Human Cannabinoid Receptor I aa 320-334 (internal sequence). (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1)
    Sequence:

    HTSEDGKVQVTRPDQ

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab40860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa).

65kDa band detected in rat brain lysate.The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343).

Target

Images

  • Western blot - Anti-Cannabinoid Receptor I antibody (ab40860)
    Western blot - Anti-Cannabinoid Receptor I antibody (ab40860)
    Anti-Cannabinoid Receptor I antibody (ab40860) at 0.1 µg/ml + Rat brain tissue lysate (35µg protein in RIPA buffer)

    Predicted band size: 53 kDa



    Primary incubation was 1 hour.

    Detected by chemiluminescence.

References

This product has been referenced in:

  • Kim J  et al. Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid system gene expression in proliferating and differentiating C2C12 myoblasts. Front Physiol 5:100 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24711795) »
See 1 Publication for this product

Publishing research using ab40860? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews

Flow Cytometry abreview for Anti-Cannabinoid Receptor I antibody

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (synovial fibroblasts from osteoarthritic person)
Specification
synovial fibroblasts from osteoarthritic person
Preparation
Cell harvesting/tissue preparation method: cells were detached by accutase (PAA), spun and fixed in 4 % formalin for 20 min. Then, cells were spun and resuspended in 100 µl BD Perm/Wash solution containing 0,5 µg CB1 (ab40860). This was then incubated for 30 min at 4 degrees. Samples were washed with 1ml of BD Perm/Wash buffer and spun again. Then 100 µl of BD Perm/wash were added including 1 µl secondary antibody (donkey anti goat, Santa Cruz Biotech). This was again incubated at 4 degrees for 30 min. After another wash step, cells were resuspended in 4 % Formalin in PBS and analyzed.
Sample buffer: BD Perm/Wash, 4 % Formaldehyde in PBS
Fixation
Formaldehyde
Permeabilization
Yes - BD Perm/Wash
Gating Strategy
fibroblast culture only
Read More

Dr. Torsten Lowin

Verified customer

Submitted Apr 16 2009

Western blot abreview for Anti-Cannabinoid Receptor I antibody

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Chinese Hamster Recombinant protein (CHO cells expressing human CB1 receptor)
Loading amount
60000 cells
Specification
CHO cells expressing human CB1 receptor
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Nov 26 2008

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