RecombinantRabMAb

Recombinant Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

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Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Flow Cytometry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I
  • Suitable for: WB, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cannabinoid Receptor I antibody [EPR23934-20]
    See all Cannabinoid Receptor I primary antibodies

  • Description

    Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Cannabinoid Receptor I aa 300-400. The exact sequence is proprietary.
    Database link: P21554

  • Positive control

    • WB: Human brain and hippocampus tissue lysate; Mouse brain tissue lysate; Rat brain tissue lysate; SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate; NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. ICC: SH-SY5Y cells; mouse primary neuron; rat primary neuron. Flow Cyt: SH-SY5Y cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab259323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 53 kDa.
ICC 1/100.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for IHC-Fr,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human hippocampus tissue lysate
      Lane 3 : Mouse brain tissue lysate
      Lane 4 : Rat brain tissue lysate

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

      Predicted band size: 53 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: Lane 1: 3 minutes; Lanes 2-4: 70 seconds.

       

    • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling Cannabinoid receptor 1 with ab259323 at 1/100 (5.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Flow Cytometry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      Flow Cytometry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

      Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP secondary antibody(HRP))

      Predicted band size: 53 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: Lane 1: 67 seconds; Lane 2: 3 minutes.

    • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse liver tissue lysate

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

      Predicted band size: 53 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The expression profile observed is consistent with what has been described in the literature (PMID: 9655502).

      Negative control: mouse liver (PMID: 9655502).

       

      Exposure time: 70 seconds

    • Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
      Immunocytochemistry - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab259323? Please let us know so that we can cite the reference in this datasheet.

    ab259323 has not yet been referenced specifically in any publications.

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