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  1. Link

    cannabinoid-receptor-i-antibody-epr23934-20-ab259323.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Cannabinoid Receptor
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RecombinantRabMAb

Recombinant Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

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  • SDS
  • Certificate of Compliance
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Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Flow Cytometry (Intracellular) - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-Cannabinoid Receptor I antibody [EPR23934-20]
    See all Cannabinoid Receptor I primary antibodies
  • Description

    Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), ICC/IFmore details
    Unsuitable for: IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain and hippocampus tissue lysate; Mouse brain tissue lysate; Rat brain tissue lysate; SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate; NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. ICC/IF: SH-SY5Y cells; mouse primary neuron; rat primary neuron. Flow Cyt (intra): SH-SY5Y cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23934-20
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Cannabinoid Receptor
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Cannabinoid Receptor I antibody [EPR23934-20] - BSA and Azide free (ab278508)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Anti-MAP2 antibody [HM-2] (ab11267)
    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab259323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 53 kDa.
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.
Notes
WB
1/1000. Predicted molecular weight: 53 kDa.
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.
Application notes
Is unsuitable for IHC-Fr,IHC-P or IP.

Target

  • Function

    Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P21554 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1268 Human
    • Entrez Gene: 12801 Mouse
    • Entrez Gene: 25248 Rat
    • Omim: 114610 Human
    • SwissProt: P21554 Human
    • SwissProt: P47746 Mouse
    • SwissProt: P20272 Rat
    • Unigene: 709067 Human
    • Unigene: 75110 Human
    • Unigene: 7992 Mouse
    • Unigene: 222240 Rat
    • Unigene: 89774 Rat
    see all
  • Alternative names

    • CANN6 antibody
    • Cannabinoid receptor 1 antibody
    • CB-R antibody
    • CB1 antibody
    • CB1A antibody
    • CB1K5 antibody
    • CB1R antibody
    • Central cannabinoid receptor antibody
    • CNR antibody
    • CNR1 antibody
    • CNR1_HUMAN antibody
    • OTTHUMP00000016838 antibody
    • OTTHUMP00000214579 antibody
    see all

Images

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human hippocampus tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1: 3 minutes; Lanes 2-4: 70 seconds.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling Cannabinoid receptor 1 with ab259323 at 1/100 (5.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    Flow Cytometry (Intracellular) - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1: 67 seconds; Lane 2: 3 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used as counterstain at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 53 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 9655502).

    Negative control: mouse liver (PMID: 9655502).

     

    Exposure time: 70 seconds

  • Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
    Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used as counterstain at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab259323? Please let us know so that we can cite the reference in this datasheet.

ab259323 has not yet been referenced specifically in any publications.

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