Recombinant Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I
- Suitable for: WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Cannabinoid Receptor I antibody [EPR23934-20]
See all Cannabinoid Receptor I primary antibodies -
Description
Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human brain and hippocampus tissue lysate; Mouse brain tissue lysate; Rat brain tissue lysate; SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate; NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. ICC/IF: SH-SY5Y cells; mouse primary neuron; rat primary neuron. Flow Cyt (intra): SH-SY5Y cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23934-20 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 53 kDa.
|
|
Flow Cyt (Intra) |
1/500.
|
|
ICC/IF |
1/100.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 53 kDa. |
Flow Cyt (Intra)
1/500. |
ICC/IF
1/100. |
Target
-
Function
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: P21554 Human
- SwissProt: P47746 Mouse
- SwissProt: P20272 Rat
- Unigene: 709067 Human
see all -
Alternative names
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
Images
-
All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 53 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1: 3 minutes; Lanes 2-4: 70 seconds.
-
Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling Cannabinoid receptor 1 with ab259323 at 1/100 (5.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
-
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
-
All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 53 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1: 67 seconds; Lane 2: 3 minutes.
-
Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used as counterstain at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
-
All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 53 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 9655502).
Negative control: mouse liver (PMID: 9655502).
Exposure time: 70 seconds
-
Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used as counterstain at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab259323 has not yet been referenced specifically in any publications.